PMID- 10090149
OWN - NLM
STAT- MEDLINE
DCOM- 19990528
LR  - 20180111
IS  - 1058-8388 (Print)
IS  - 1058-8388 (Linking)
VI  - 214
IP  - 3
DP  - 1999 Mar
TI  - The LIM protein, CRP1, is a smooth muscle marker.
PG  - 229-38
AB  - LIM domains are double zinc-finger motifs found in many proteins that play
      central roles in cell differentiation. Members of the cysteine-rich protein (CRP)
      family display two LIM domains and are implicated in muscle development. Here we 
      describe the characterization of one member of this family, CRP1, in the mouse.
      We have isolated and sequenced murine cDNAs that encode CRP1. We have determined 
      by Northern analysis and in situ hybridization that CRP1 expression is
      developmentally regulated in the embryonic mouse and displays organ specific
      regulation in adults. The gene encoding CRP1 is expressed in the smooth muscle
      cells (SMCs) of the dorsal aorta at E9.5, thus illustrating that CRP1 is an early
      marker for SMC differentiation at that site. As development proceeds, CRP1
      transcripts are observed throughout the SMC lineage, with minimal, transient
      expression detected in skeletal and cardiac muscle. Interestingly, although
      several markers of mature smooth muscle are already expressed, CRP1 expression in
      the bladder is not upregulated until the onset of bladder expansion at embryonic 
      day 16.5, at which time its expression becomes very prominent. CRP1 expression
      persists into adulthood with prominent expression observed in both vascular and
      visceral smooth muscle. The results reported here define CRP1 as a general marker
      of smooth muscle lineages.
FAU - Henderson, J R
AU  - Henderson JR
AD  - Department of Biology, University of Utah, Salt Lake City 84112-0840, USA.
FAU - Macalma, T
AU  - Macalma T
FAU - Brown, D
AU  - Brown D
FAU - Richardson, J A
AU  - Richardson JA
FAU - Olson, E N
AU  - Olson EN
FAU - Beckerle, M C
AU  - Beckerle MC
LA  - eng
SI  - GENBANK/AF092921
GR  - R01 HL060591/HL/NHLBI NIH HHS/United States
GR  - HL60591/HL/NHLBI NIH HHS/United States
GR  - CA42014/CA/NCI NIH HHS/United States
GR  - 5T32 HD07491/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Dev Dyn
JT  - Developmental dynamics : an official publication of the American Association of
      Anatomists
JID - 9201927
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Avian Proteins)
RN  - 0 (Biomarkers)
RN  - 0 (Carrier Proteins)
RN  - 0 (Csrp1 protein, mouse)
RN  - 0 (DNA, Complementary)
RN  - 0 (LIM Domain Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (cysteine and glycine-rich protein 3)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Avian Proteins
MH  - Base Sequence
MH  - Biomarkers
MH  - Carrier Proteins/*genetics
MH  - DNA, Complementary
MH  - *Gene Expression Regulation, Developmental
MH  - LIM Domain Proteins
MH  - Mice
MH  - Molecular Sequence Data
MH  - Muscle Proteins/*genetics
MH  - Muscle, Smooth/*embryology
MH  - *Nuclear Proteins
MH  - Urinary Bladder
EDAT- 1999/03/25 03:02
MHDA- 2000/06/22 10:00
CRDT- 1999/03/25 03:02
PHST- 1999/03/25 03:02 [pubmed]
PHST- 2000/06/22 10:00 [medline]
PHST- 1999/03/25 03:02 [entrez]
AID - 10.1002/(SICI)1097-0177(199903)214:3<229::AID-AJA6>3.0.CO;2-S [pii]
AID - 10.1002/(SICI)1097-0177(199903)214:3<229::AID-AJA6>3.0.CO;2-S [doi]
PST - ppublish
SO  - Dev Dyn. 1999 Mar;214(3):229-38. doi:
      10.1002/(SICI)1097-0177(199903)214:3<229::AID-AJA6>3.0.CO;2-S.