PMID- 10090148
OWN - NLM
STAT- MEDLINE
DCOM- 19990528
LR  - 20071114
IS  - 1058-8388 (Print)
IS  - 1058-8388 (Linking)
VI  - 214
IP  - 3
DP  - 1999 Mar
TI  - Alpha9 and beta8 integrin expression correlates with the merger of the developing
      mouse eyelids.
PG  - 216-28
AB  - As previously reported, alpha9 integrin is expressed between the merged or fused 
      eyelids of mice at birth, and changes in alpha9 localization occur during lid
      opening. To determine whether alpha9 and/or additional integrin subunits mediate 
      the emergence and temporary fusion of the eyelids, immunofluorescence and
      confocal microscopy were used to evaluate the localization of various integrin
      subunits in the developing ocular surface of the mouse. No detectable beta5,
      beta6, or beta7 integrins were observed on the epithelia of the ocular surface.
      alpha2, alpha3, alphav, and beta1 integrins were most abundant in the basal cells
      beginning at 13.5 days post conception and remained primarily localized to the
      basal cell layers throughout development. beta4 was localized at the basal
      surface of the epidermal basal cells beginning at 13.5 days post conception but
      was not found on the corneal epithelial basal cells until after birth. alpha9 and
      beta8 integrins were present on suprabasal cells of the epidermis at the leading 
      edge of the eyelid before merger and on the epithelial bridge that forms
      immediately after these tissues merge, suggesting that they play a role in the
      initial fusion of the epithelial tissues of the eyelid and in stabilizing the
      epithelial junction. After birth and into adulthood, beta8 was retained within
      the suprabasal cell layers of the epidermis, whereas alpha9 became localized to
      the basal cells of the epidermis, the conjunctiva, and the limbus. The lack of
      co-localization of beta4 with either alpha9 or beta8 in double-labeling studies
      suggests that alpha9 and beta8 are restricted to the lateral and apical aspects
      of those cells in which they are expressed. The presence of tenascin-C and
      laminin-5 at the epithelial junction site suggests that alpha9: tenascin-C and
      beta4: laminin-5 interactions may play a role in stabilizing the fusion between
      lids early on but do not appear to be involved in the movement of the lids across
      the cornea. The data presented identify specific integrins and matrix proteins
      that are likely to mediate eyelid fusion.
FAU - Stepp, M A
AU  - Stepp MA
AD  - Department of Anatomy and Cell Biology, Department of Ophthalmology, The George
      Washington University Medical Center, Washington, DC 20037, USA. mastepp@gwu.edu
LA  - eng
GR  - R01-EYO8512-09/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Dev Dyn
JT  - Developmental dynamics : an official publication of the American Association of
      Anatomists
JID - 9201927
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Integrin alpha Chains)
RN  - 0 (Integrin beta Chains)
RN  - 0 (Integrins)
RN  - 0 (Tenascin)
RN  - 0 (integrin alpha9)
RN  - 0 (integrin beta8)
RN  - 0 (kalinin)
SB  - IM
MH  - Animals
MH  - Cell Adhesion Molecules/genetics
MH  - Eyelids/*embryology
MH  - Female
MH  - *Gene Expression Regulation, Developmental
MH  - *Integrin alpha Chains
MH  - *Integrin beta Chains
MH  - Integrins/*genetics
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Tenascin/genetics
EDAT- 1999/03/25 03:02
MHDA- 2000/06/22 10:00
CRDT- 1999/03/25 03:02
PHST- 1999/03/25 03:02 [pubmed]
PHST- 2000/06/22 10:00 [medline]
PHST- 1999/03/25 03:02 [entrez]
AID - 10.1002/(SICI)1097-0177(199903)214:3<216::AID-AJA5>3.0.CO;2-4 [pii]
AID - 10.1002/(SICI)1097-0177(199903)214:3<216::AID-AJA5>3.0.CO;2-4 [doi]
PST - ppublish
SO  - Dev Dyn. 1999 Mar;214(3):216-28. doi:
      10.1002/(SICI)1097-0177(199903)214:3<216::AID-AJA5>3.0.CO;2-4.