PMID- 10089883
OWN - NLM
STAT- MEDLINE
DCOM- 19990422
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 96
IP  - 5
DP  - 1999 Mar 5
TI  - EGF receptor signaling stimulates SRC kinase phosphorylation of clathrin,
      influencing clathrin redistribution and EGF uptake.
PG  - 677-87
AB  - Epidermal growth factor (EGF) binding to its receptor causes rapid
      phosphorylation of the clathrin heavy chain at tyrosine 1477, which lies in a
      domain controlling clathrin assembly. EGF-mediated clathrin phosphorylation is
      followed by clathrin redistribution to the cell periphery and is the product of
      downstream activation of SRC kinase by EGF receptor (EGFR) signaling. In cells
      lacking SRC kinase, or cells treated with a specific SRC family kinase inhibitor,
      EGF stimulation of clathrin phosphorylation and redistribution does not occur,
      and EGF endocytosis is delayed. These observations demonstrate a role for SRC
      kinase in modification and recruitment of clathrin during ligand-induced EGFR
      endocytosis and thereby define a novel effector mechanism for regulation of
      endocytosis by receptor signaling.
FAU - Wilde, A
AU  - Wilde A
AD  - Department of Immunology and Microbiology, University of California, San
      Francisco 94143-0552, USA.
FAU - Beattie, E C
AU  - Beattie EC
FAU - Lem, L
AU  - Lem L
FAU - Riethof, D A
AU  - Riethof DA
FAU - Liu, S H
AU  - Liu SH
FAU - Mobley, W C
AU  - Mobley WC
FAU - Soriano, P
AU  - Soriano P
FAU - Brodsky, F M
AU  - Brodsky FM
LA  - eng
GR  - GM38093/GM/NIGMS NIH HHS/United States
GR  - GM57657/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Clathrin)
RN  - 0 (Ligands)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 2.7.10.2 (Proto-Oncogene Proteins pp60(c-src))
RN  - EC 2.7.10.2 (src-Family Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Biological Transport
MH  - Cattle
MH  - Clathrin/*metabolism
MH  - Endocytosis/*drug effects
MH  - Epidermal Growth Factor/*metabolism
MH  - ErbB Receptors/*physiology
MH  - Humans
MH  - Ligands
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phosphorylation/drug effects
MH  - Protein Processing, Post-Translational/*drug effects
MH  - Proto-Oncogene Proteins pp60(c-src)/metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Tumor Cells, Cultured
MH  - src-Family Kinases/*metabolism
EDAT- 1999/03/25 00:00
MHDA- 1999/03/25 00:01
CRDT- 1999/03/25 00:00
PHST- 1999/03/25 00:00 [pubmed]
PHST- 1999/03/25 00:01 [medline]
PHST- 1999/03/25 00:00 [entrez]
AID - S0092-8674(00)80578-4 [pii]
AID - 10.1016/s0092-8674(00)80578-4 [doi]
PST - ppublish
SO  - Cell. 1999 Mar 5;96(5):677-87. doi: 10.1016/s0092-8674(00)80578-4.