PMID- 10089880
OWN - NLM
STAT- MEDLINE
DCOM- 19990422
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 96
IP  - 5
DP  - 1999 Mar 5
TI  - Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of
      novel polyubiquitin chains for DNA repair.
PG  - 645-53
AB  - Ubiquitin-conjugating enzyme variant (UEV) proteins resemble
      ubiquitin-conjugating enzymes (E2s) but lack the defining E2 active-site residue.
      The MMS2-encoded UEV protein has been genetically implicated in error-free
      postreplicative DNA repair in Saccharomyces cerevisiae. We show that Mms2p forms 
      a specific heteromeric complex with the UBC13-encoded E2 and is required for the 
      Ubc13p-dependent assembly of polyubiquitin chains linked through lysine 63. A
      ubc13 yeast strain is UV sensitive, and single, double, and triple mutants of the
      UBC13, MMS2, and ubiquitin (ubiK63R) genes display a comparable phenotype. These 
      findings support a model in which an Mms2p/Ubc13p complex assembles novel
      polyubiquitin chains for signaling in DNA repair, and they suggest that UEV
      proteins may act to increase diversity and selectivity in ubiquitin conjugation.
FAU - Hofmann, R M
AU  - Hofmann RM
AD  - Department of Biochemistry, School of Public Health, Johns Hopkins University,
      Baltimore, Maryland 21205, USA.
FAU - Pickart, C M
AU  - Pickart CM
LA  - eng
GR  - DK46984/DK/NIDDK NIH HHS/United States
GR  - T32 ES07141/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Biopolymers)
RN  - 0 (Fungal Proteins)
RN  - 0 (MMS2 protein, S cerevisiae)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Ubiquitins)
RN  - EC 2.3.2.23 (Ubiquitin-Conjugating Enzymes)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 6.- (Ligases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Biopolymers/metabolism
MH  - Cattle
MH  - DNA Repair/*physiology
MH  - Fungal Proteins/*physiology
MH  - Humans
MH  - Ligases/*physiology
MH  - Macromolecular Substances
MH  - Molecular Sequence Data
MH  - Multigene Family
MH  - Recombinant Fusion Proteins/physiology
MH  - Saccharomyces cerevisiae/genetics/*physiology
MH  - *Saccharomyces cerevisiae Proteins
MH  - Species Specificity
MH  - Ubiquitin-Conjugating Enzymes
MH  - Ubiquitin-Protein Ligases
MH  - Ubiquitins/*metabolism
EDAT- 1999/03/25 00:00
MHDA- 1999/03/25 00:01
CRDT- 1999/03/25 00:00
PHST- 1999/03/25 00:00 [pubmed]
PHST- 1999/03/25 00:01 [medline]
PHST- 1999/03/25 00:00 [entrez]
AID - S0092-8674(00)80575-9 [pii]
AID - 10.1016/s0092-8674(00)80575-9 [doi]
PST - ppublish
SO  - Cell. 1999 Mar 5;96(5):645-53. doi: 10.1016/s0092-8674(00)80575-9.