PMID- 10088603
OWN - NLM
STAT- MEDLINE
DCOM- 19990325
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 65
IP  - 2
DP  - 1999 Feb
TI  - Granulocyte-macrophage colony-stimulating factor modulates tapasin expression in 
      human neutrophils.
PG  - 205-10
AB  - Differential display-polymerase chain reaction (DD-PCR) was used to evaluate 
      changes in mRNA expression of granulocyte-macrophage colony-stimulating factor 
      (GM-CSF) treated human neutrophils to better understand how this cytokine affects 
      the functions of neutrophils at the molecular level. Although a variety of cDNA 
      fragments were identified as modulated by GM-CSF with the use of DD-PCR, one 
      fragment in particular, NGS-17 (neutrophil GM-CSF-stimulated fragment #17), was 
      characterized. The NGS-17 fragment hybridized to a 3.8-kh mRNA that encodes for a 
      protein of a predicted molecular mass of 47.6 kDa. After cloning and sequencing, 
      this gene was found to code for the recently sequenced tapasin or TAP-A protein. 
      Immunoprecipitation and immunoblotting studies using anti-tapasin antibodies 
      showed that tapasin is expressed in neutrophils and is associated with the MHC 
      class I-TAP complex. Moreover, tapasin expression was found to be induced by 
      dimethyl sulfoxide and by retinoic acid in HL-60 cells. This is the first report 
      on the expression of tapasin in human neutrophils. It provides novel information, 
      at the molecular level, on how GM-CSF enhances the functions of these cells.
FAU - El Ouakfaoui, S
AU  - El Ouakfaoui S
AD  - Laboratoire de Recherche sur l'Arthrite, Centre de Recherche du Centre 
      Hospitalier de l'Universite Laval, Quebec, Canada.
FAU - Heitz, D
AU  - Heitz D
FAU - Paquin, R
AU  - Paquin R
FAU - Beaulieu, A D
AU  - Beaulieu AD
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Antiporters)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Immunoglobulins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (tapasin)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Antiporters/genetics/*metabolism
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/*pharmacology
MH  - HL-60 Cells/drug effects
MH  - Histocompatibility Antigens Class I/*metabolism
MH  - Humans
MH  - Immunoglobulins/genetics/*metabolism
MH  - Membrane Transport Proteins
MH  - Neutrophils/*drug effects/metabolism
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/metabolism
EDAT- 1999/03/24 00:00
MHDA- 1999/03/24 00:01
CRDT- 1999/03/24 00:00
PHST- 1999/03/24 00:00 [pubmed]
PHST- 1999/03/24 00:01 [medline]
PHST- 1999/03/24 00:00 [entrez]
AID - 10.1002/jlb.65.2.205 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1999 Feb;65(2):205-10. doi: 10.1002/jlb.65.2.205.