PMID- 10087504
OWN - NLM
STAT- MEDLINE
DCOM- 19990629
LR  - 20191103
IS  - 0968-7688 (Print)
IS  - 0968-7688 (Linking)
VI  - 15
IP  - 4
DP  - 1998 Oct-Dec
TI  - Preliminary characterization of the protein encoded by human testis-enhanced gene
      transcript (TEGT).
PG  - 177-87
AB  - TEGT is a conserved, widely expressed gene transcript of unknown function that
      has been studied previously only at the nucleic acid level. The deduced amino
      acid sequence predicts a highly hydrophobic, 26.5 kDa integral membrane protein
      with seven potential transmembrane domains. Little else is known about TEGT
      protein because of the lack of definitive homology to other known sequences and
      the absence of informative consensus motifs. The present report details a
      preliminary study of human TEGT (hTEGT) protein. (i) In vitro translation of
      hTEGT in reticulocyte lysates required the presence of microsomes for efficient
      synthesis, suggesting that hTEGT must target to the endoplasmic reticulum to be
      translated. Immunofluorescence of cells transiently expressing
      haemagglutinin-tagged hTEGT localized the protein mainly to the endoplasmic
      reticulum. The protein demonstrated no obvious post-translational modifications
      such as signal-peptide cleavage, N-linked glycosylation or O-linked
      glycosylation. (ii) Both hTEGT and haemagglutinin-tagged hTEGT appeared to retain
      partial secondary and tertiary structure in the presence of SDS. Both
      electrophoresed as a broad band or doublet with apparent molecular weights of
      22-24.5 kDa on SDS-PAGE, aggregated either homotypically or heterotypically when 
      boiled in SDS, and were toxic after 24 h when highly overexpressed in 293 T
      cells. These properties are believed to be caused by the protein's
      hydrophobicity. (iii) The protein appeared to associate strongly with other
      intracellular molecules since haemagglutinin-tagged hTEGT was extracted poorly
      from transiently transfected HeLa cells. Further study will be required to
      determine the cellular function of TEGT.
FAU - Cowling, R T
AU  - Cowling RT
AD  - Cancer Research Group, Ottawa Regional Cancer Centre, Ontario, Canada.
FAU - Birnboim, H C
AU  - Birnboim HC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mol Membr Biol
JT  - Molecular membrane biology
JID - 9430797
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (Proteins)
RN  - 0 (TMBIM6 protein, human)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - YOW8V9698H (Dimethyl Sulfoxide)
SB  - IM
MH  - Apoptosis Regulatory Proteins
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Cell Line
MH  - Dactinomycin/pharmacology
MH  - Dimethyl Sulfoxide/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Endoplasmic Reticulum/metabolism
MH  - Fluorescent Antibody Technique
MH  - HL-60 Cells
MH  - Humans
MH  - Male
MH  - Membrane Proteins
MH  - Mutagenesis, Site-Directed
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - Proteins/*chemistry/genetics
MH  - Temperature
MH  - Time Factors
MH  - Transcription, Genetic
MH  - Transfection
EDAT- 1999/03/24 00:00
MHDA- 1999/03/24 00:01
CRDT- 1999/03/24 00:00
PHST- 1999/03/24 00:00 [pubmed]
PHST- 1999/03/24 00:01 [medline]
PHST- 1999/03/24 00:00 [entrez]
AID - 10.3109/09687689709044319 [doi]
PST - ppublish
SO  - Mol Membr Biol. 1998 Oct-Dec;15(4):177-87. doi: 10.3109/09687689709044319.