PMID- 10087197
OWN - NLM
STAT- MEDLINE
DCOM- 19990524
LR  - 20191210
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 56
IP  - 3
DP  - 1999 Mar 15
TI  - The genomic organization of type I keratin genes in mice.
PG  - 303-9
AB  - We isolated two new keratin cDNAs by screening a cDNA library constructed from
      poly(A)+ RNA of the dorsal and abdominal skin of C57BL/10J mice with a probe of
      human KRT14. Due to its high sequence homology to human keratin 17 cDNA, one
      full-length cDNA is most likely to be mouse keratin 17 (Krt1-17) cDNA. The other 
      is the putative full-length cDNA of a novel type I keratin gene, designated
      Krt1-c29. These two keratin genes were mapped to the distal portion of Chromosome
      11, where the mouse keratin gene complex-1 (Krt1) is localized. To elucidate the 
      genomic organization of Krt1 in mice, we carried out genetic and physical
      analyses of Krt1. A large-scale linkage analysis using intersubspecific
      backcrosses suggested that there are two major clusters in Krt1, one containing
      Krt1-c29, Krt1-10, and Krt1-12 and the other containing Krt1-14, -15, -17, and
      -19. Truncation experiments with two yeast artificial chromosome clones
      containing the two clusters above have revealed that the gene order of Krt1 is
      centromere-Krt1-c29-Krt1-10-Krt1-12-Krt1-13-K rt1-15-Krt1-19-Krt1-14-K
      rt1-17-telomere. Finally, we analyzed sequence divergence between the genes
      belonging to the Krt1 complex. The results clearly indicated that genes are
      classified into two major groups with respect to phylogenetic relationship. Each 
      group consists of the respective gene cluster demonstrated by genetic and
      physical analyses in this study, suggesting that the physical organization of the
      Krt1 complex reflects the evolutionary process of gene duplication of this
      complex.
CI  - Copyright 1999 Academic Press.
FAU - Sato, H
AU  - Sato H
AD  - Department of Ophthalmology, Tohoku University School of Medicine, Seiryo-Machi
      1-1, Sendai, 980-8574, Japan.
FAU - Koide, T
AU  - Koide T
FAU - Sagai, T
AU  - Sagai T
FAU - Ishiguro, S I
AU  - Ishiguro SI
FAU - Tamai, M
AU  - Tamai M
FAU - Saitou, N
AU  - Saitou N
FAU - Shiroishi, T
AU  - Shiroishi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Keratins, Hair-Specific)
RN  - 0 (Keratins, Type I)
RN  - 0 (Krt17 protein, mouse)
RN  - 0 (Krt27 protein, mouse)
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Northern
MH  - Blotting, Southern
MH  - Chromosomes, Artificial, Yeast
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Gene Library
MH  - Genetic Linkage
MH  - Humans
MH  - Keratins/*genetics
MH  - Keratins, Hair-Specific
MH  - Keratins, Type I
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microsatellite Repeats
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Skin/metabolism
MH  - Tissue Distribution
EDAT- 1999/03/24 00:00
MHDA- 1999/03/24 00:01
CRDT- 1999/03/24 00:00
PHST- 1999/03/24 00:00 [pubmed]
PHST- 1999/03/24 00:01 [medline]
PHST- 1999/03/24 00:00 [entrez]
AID - S0888-7543(98)95721-4 [pii]
AID - 10.1006/geno.1998.5721 [doi]
PST - ppublish
SO  - Genomics. 1999 Mar 15;56(3):303-9. doi: 10.1006/geno.1998.5721.