PMID- 10086359
OWN - NLM
STAT- MEDLINE
DCOM- 19990329
LR  - 20161124
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 398
IP  - 6723
DP  - 1999 Mar 11
TI  - CBP-independent activation of CREM and CREB by the LIM-only protein ACT.
PG  - 165-9
AB  - Transcriptional activation by CREB and CREM requires phosphorylation of a serine 
      residue within the activation domain (Ser 133 in CREB; Ser 117 in CREM) which as 
      a result interacts with the coactivator CBP. The activator CREM is highly
      expressed in male germ cells and is required for post-meiotic gene expression.
      Using a two-hybrid screen, we have isolated a testis-derived complementary DNA
      encoding a protein that we term ACT (for activator of CREM in testis), a LIM-only
      protein which specifically associates with CREM. ACT is expressed coordinately
      with CREM in a tissue- and developmentally regulated manner. It strongly
      stimulates CREM transcriptional activity in yeast and mammalian cells and
      contains an intrinsic activation function. As ACT bypasses the classical
      requirements for activation, namely phosphorylation of Ser 117 and interaction
      with CBP, it represents a new route for transcriptional activation by CREM and
      CREB. ACT may define a previously undiscovered class of tissue-specific
      coactivators whose function could be specific for distinct cellular
      differentiation programmes.
FAU - Fimia, G M
AU  - Fimia GM
AD  - Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS-INSERM-ULP, 
      Illkirch, Strasbourg, France.
FAU - De Cesare, D
AU  - De Cesare D
FAU - Sassone-Corsi, P
AU  - Sassone-Corsi P
LA  - eng
SI  - GENBANK/AF083394
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Fhl5 protein, mouse)
RN  - 0 (LIM Domain Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (activator of CREM in testis (ACT))
RN  - 135844-64-3 (Cyclic AMP Response Element Modulator)
RN  - 452VLY9402 (Serine)
RN  - EC 2.3.1.48 (CREB-Binding Protein)
RN  - EC 2.3.1.48 (Crebbp protein, mouse)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - COS Cells
MH  - CREB-Binding Protein
MH  - Cyclic AMP Response Element Modulator
MH  - Cyclic AMP Response Element-Binding Protein/*metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - LIM Domain Proteins
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/*metabolism
MH  - Phosphorylation
MH  - Protein Binding
MH  - Recombinant Fusion Proteins/metabolism
MH  - *Repressor Proteins
MH  - Serine/metabolism
MH  - Testis/metabolism
MH  - Trans-Activators/*metabolism
MH  - Transcription Factors/genetics/*metabolism
MH  - Yeasts
MH  - Zinc Fingers
EDAT- 1999/03/23 03:03
MHDA- 2001/03/23 10:01
CRDT- 1999/03/23 03:03
PHST- 1999/03/23 03:03 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/03/23 03:03 [entrez]
AID - 10.1038/18237 [doi]
PST - ppublish
SO  - Nature. 1999 Mar 11;398(6723):165-9. doi: 10.1038/18237.