PMID- 10085160
OWN - NLM
STAT- MEDLINE
DCOM- 19990429
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 13
DP  - 1999 Mar 26
TI  - PU.1 and USF are required for macrophage-specific mannose receptor promoter
      activity.
PG  - 9098-107
AB  - In the current study we report the isolation of 854 base pairs of the rat mannose
      receptor promoter. Analysis of the sequence revealed one Sp1 site, three PU.1
      sites, and a potential TATA box (TTTAAA) 33 base pairs 5' of the transcriptional 
      start site. The tissue specificity of the promoter was determined using transient
      transfections. The promoter was most active in the mature macrophage cell line
      NR8383 although the promoter also showed activity in the monocytic cell line RAW.
      No activity was observed in pre-monocytic cell lines or epithelial cell lines.
      Mutation of the TTTAAA sequence to TTGGAA resulted in a 50% decrease in activity 
      in transient transfection assays suggesting that the promoter contains a
      functional TATA box. Using electrophoretic mobility shift assays and mutagenesis 
      we established that the transcription factors Sp1, PU.1, and USF bound to the
      mannose receptor promoter, but only PU.1 and USF contributed to activation.
      Transient transfections using a dominant negative construct of USF resulted in a 
      50% decrease in mannose receptor promoter activity, further establishing the role
      of USF in activating the rat mannose receptor promoter. Comparison of the rat,
      mouse, and human sequence demonstrated that some binding sites are not conserved.
      Gel shifts were performed to investigate differences in protein binding between
      species. USF bound to the rat and human promoter but not to the mouse promoter,
      suggesting that different mechanisms are involved in regulation of mannose
      receptor expression in these species. From these results we conclude that,
      similar to other myeloid promoters, transcription of the rat mannose receptor is 
      regulated by binding of PU.1 and a ubiquitous factor at an adjacent site.
      However, unlike other myeloid promoters, we have identified USF as the ubiquitous
      factor, and demonstrated that the promoter contains a functional TATA box.
FAU - Egan, B S
AU  - Egan BS
AD  - Department of Biochemistry, Vanderbilt University, Nashville, Tennessee 37212,
      USA.
FAU - Lane, K B
AU  - Lane KB
FAU - Shepherd, V L
AU  - Shepherd VL
LA  - eng
SI  - GENBANK/AF121966
GR  - EY02853/EY/NEI NIH HHS/United States
GR  - HL55977/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Mannose-Binding Lectins)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Sp1 Transcription Factor)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (Upstream Stimulatory Factors)
RN  - 0 (Usf1 protein, rat)
RN  - 0 (mannose receptor)
RN  - 0 (proto-oncogene protein Spi-1)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - DNA-Binding Proteins/analysis
MH  - Genes, Reporter/genetics
MH  - *Lectins, C-Type
MH  - Macrophages, Alveolar/*metabolism
MH  - *Mannose-Binding Lectins
MH  - Molecular Sequence Data
MH  - Oligodeoxyribonucleotides/genetics
MH  - Promoter Regions, Genetic/*genetics
MH  - Proto-Oncogene Proteins/*genetics
MH  - Rats
MH  - Receptors, Cell Surface/*genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Nucleic Acid
MH  - Sp1 Transcription Factor/genetics
MH  - Trans-Activators/*genetics
MH  - Transcription Factors/*genetics
MH  - Transcription, Genetic/genetics
MH  - Transfection/genetics
MH  - Upstream Stimulatory Factors
EDAT- 1999/03/20 00:00
MHDA- 1999/03/20 00:01
CRDT- 1999/03/20 00:00
PHST- 1999/03/20 00:00 [pubmed]
PHST- 1999/03/20 00:01 [medline]
PHST- 1999/03/20 00:00 [entrez]
AID - 10.1074/jbc.274.13.9098 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Mar 26;274(13):9098-107. doi: 10.1074/jbc.274.13.9098.