PMID- 10085102
OWN - NLM
STAT- MEDLINE
DCOM- 19990429
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 13
DP  - 1999 Mar 26
TI  - Peptide-bound major histocompatibility complex class I molecules associate with
      tapasin before dissociation from transporter associated with antigen processing.
PG  - 8649-54
AB  - Major histocompatibility complex (MHC) class I molecules present antigenic
      peptides to CD8 T cells. The peptides are generated in the cytosol, then
      translocated across the membrane of the endoplasmic reticulum by the transporter 
      associated with antigen processing (TAP). TAP is a trimeric complex consisting of
      TAP1, TAP2, and tapasin (TAP-A) as indicated for human cells by reciprocal
      coprecipitation with anti-TAP1/2 and anti-tapasin antibodies, respectively. TAP1 
      and TAP2 are required for the peptide transport. Tapasin is involved in the
      association of class I with TAP and in the assembly of class I with peptide. The 
      mechanisms of tapasin function are still unknown. Moreover, there has been no
      evidence for a murine tapasin analogue, which has led to the suggestion that
      murine MHC class I binds directly to TAP1/2. In this study, we have cloned the
      mouse analogue of tapasin. The predicted amino acid sequence showed 78% identity 
      to human tapasin with identical consensus sequences of signal peptide, N-linked
      glycosylation site, transmembrane domain and double lysine motif. However, there 
      was less homology (47%) found at the predicted cytosolic domain, and in addition,
      mouse tapasin is 14 amino acids longer than the human analogue at the C terminus.
      This part of the molecule may determine the species specificity for interaction
      with MHC class I or TAP1/2. Like human tapasin, mouse tapasin binds both to
      TAP1/2 and MHC class I. In TAP2-mutated RMA-S cells, both TAP1 and MHC class I
      were coprecipitated by anti-tapasin antiserum indicative of association of
      tapasin with TAP1 but not TAP2. With crosslinker-modified peptides and purified
      microsomes, anti-tapasin coprecipitated both peptide-bound MHC class I and
      TAP1/2. In contrast, anti-calreticulin only coprecipitated peptide-free MHC class
      I molecules. This difference in association with peptide-loaded class I suggests 
      that tapasin functions later than calreticulin during MHC class I assembly, and
      controls peptide loading onto MHC class I molecules in the endoplasmic reticulum.
FAU - Li, S
AU  - Li S
AD  - Tumor Immunology, Lund University, Box 7031, S-22007 Lund, Sweden.
      su-ling.li@wblab.lu.se
FAU - Paulsson, K M
AU  - Paulsson KM
FAU - Sjogren, H O
AU  - Sjogren HO
FAU - Wang, P
AU  - Wang P
LA  - eng
SI  - GENBANK/AF106278
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 2)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 3)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Antiporters)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Calreticulin)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Immunoglobulins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Peptides)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (TAP1 protein, human)
RN  - 0 (Tap1 protein, mouse)
RN  - 0 (Tap2 protein, mouse)
RN  - 0 (tapasin)
RN  - 145892-13-3 (TAP2 protein, human)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 2
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 3
MH  - ATP-Binding Cassette Transporters/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigen Presentation/*immunology
MH  - Antiporters/*genetics/metabolism
MH  - Calcium-Binding Proteins/metabolism
MH  - Calreticulin
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Glycosylation
MH  - Histocompatibility Antigens Class I/*metabolism
MH  - Immunoglobulins/*genetics/metabolism
MH  - Membrane Transport Proteins
MH  - Mice
MH  - Molecular Sequence Data
MH  - Peptides/immunology
MH  - Protein Binding/immunology
MH  - Ribonucleoproteins/metabolism
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
EDAT- 1999/03/20 00:00
MHDA- 1999/03/20 00:01
CRDT- 1999/03/20 00:00
PHST- 1999/03/20 00:00 [pubmed]
PHST- 1999/03/20 00:01 [medline]
PHST- 1999/03/20 00:00 [entrez]
AID - 10.1074/jbc.274.13.8649 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Mar 26;274(13):8649-54. doi: 10.1074/jbc.274.13.8649.