PMID- 10085095
OWN - NLM
STAT- MEDLINE
DCOM- 19990429
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 13
DP  - 1999 Mar 26
TI  - Leukotriene binding, signaling, and analysis of HIV coreceptor function in mouse 
      and human leukotriene B4 receptor-transfected cells.
PG  - 8597-603
AB  - The mouse leukotriene B4 receptor (m-BLTR) gene was cloned. Membrane fractions of
      human embryonic kidney 293 cells stably expressing m-BLTR demonstrated a high
      affinity and specific binding for leukotriene B4 (LTB4, Kd = 0.24 +/- 0.03 nM).
      In competition binding experiments, LTB4 was the most potent competitor (Ki =
      0.23 +/- 0.05 nM) followed by 20-hydroxy-LTB4 (Ki = 1.1 +/- 0.2 nM) and by
      6-trans-12-epi-LTB4 and LTD4 (Ki > 1 microM). In stably transfected Chinese
      hamster ovary cells, LTB4 inhibited forskolin-activated cAMP production and
      induced an increase of intracellular calcium, suggesting that this receptor is
      coupled to Gi- and Go-like proteins. In Xenopus laevis melanophores transiently
      expressing m-BLTR, LTB4 induced the aggregation of pigment granules, confirming
      the inhibition of cAMP production induced by LTB4. BLT receptors share
      significant sequence homology with chemokine receptors (CCR5 and CXCR4) that act 
      as human immunodeficiency virus (HIV) coreceptors. However, among the 16 HIV/SIV 
      strains tested, the human BLT receptor did not act as a coreceptor for virus
      entry into CD4-expressing cells based on infection and cell-cell fusion assays.
      In 5-lipoxygenase-deficient mice, the absence of leukotriene B4 biosynthesis did 
      not detectably alter m-BLT receptor binding in membranes obtained from
      glycogen-elicited neutrophils. Isolation of the m-BLTR gene will form the basis
      of future experiments to elucidate the selective role of LTB4, as opposed to
      cysteinyl-leukotrienes, in murine models of inflammation.
FAU - Martin, V
AU  - Martin V
AD  - Center for Experimental Therapeutics, University of Pennsylvania, Philadelphia,
      Pennsylvania 19104, USA.
FAU - Ronde, P
AU  - Ronde P
FAU - Unett, D
AU  - Unett D
FAU - Wong, A
AU  - Wong A
FAU - Hoffman, T L
AU  - Hoffman TL
FAU - Edinger, A L
AU  - Edinger AL
FAU - Doms, R W
AU  - Doms RW
FAU - Funk, C D
AU  - Funk CD
LA  - eng
SI  - GENBANK/AF077673
GR  - AI40880/AI/NIAID NIH HHS/United States
GR  - HL58464/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Receptors, HIV)
RN  - 0 (Receptors, Leukotriene B4)
RN  - 1F7A44V6OU (Colforsin)
RN  - 1HGW4DR56D (Leukotriene B4)
RN  - 67526-95-8 (Thapsigargin)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 1.13.11.34 (Arachidonate 5-Lipoxygenase)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - SY7Q814VUP (Calcium)
SB  - IM
SB  - X
MH  - Animals
MH  - Arachidonate 5-Lipoxygenase/deficiency/genetics
MH  - Calcium/metabolism
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Colforsin/pharmacology
MH  - Cyclic AMP/metabolism
MH  - GTP-Binding Proteins/metabolism
MH  - Humans
MH  - Leukotriene B4/analogs & derivatives/metabolism
MH  - Melanophores/drug effects
MH  - Mice
MH  - Protein Binding
MH  - Receptors, Chemokine/metabolism
MH  - Receptors, HIV/*metabolism
MH  - Receptors, Leukotriene B4/genetics/*metabolism
MH  - Signal Transduction
MH  - Thapsigargin/pharmacology
MH  - Transfection
EDAT- 1999/03/20 00:00
MHDA- 1999/03/20 00:01
CRDT- 1999/03/20 00:00
PHST- 1999/03/20 00:00 [pubmed]
PHST- 1999/03/20 00:01 [medline]
PHST- 1999/03/20 00:00 [entrez]
AID - 10.1074/jbc.274.13.8597 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Mar 26;274(13):8597-603. doi: 10.1074/jbc.274.13.8597.