PMID- 1008494
OWN - NLM
STAT- MEDLINE
DCOM- 19770216
LR  - 20131121
IS  - 0003-4762 (Print)
IS  - 0003-4762 (Linking)
VI  - 8 Suppl 17
DP  - 1976
TI  - The binding and transport of heme by hemopexin.
PG  - 223-32
AB  - Hemopexin binds proto-, meso-, or deutero-ferriheme with high affinity, forming
      an equimolar, low-spin complex. The ferroheme-hemopexin complex, which
      coordinates with CO and readily autoxidizes, is also low-spin. Formation of the
      ferriheme-hemopexin complex requires essential histidine and tryptophan residues 
      and induces changes in the protein's tertiary structure. These changes may be
      important for the uptake of the heme-hemopexin complex by hepatocytes. Hemopexin 
      also binds other porphyrins including protoporphyrin IX, and uro- and
      coproporphyrins I and III in a 1:1 molar ratio, but they are readily displaced by
      heme and do not produce discernable changes in the protein's conformation. In
      preliminary experiments, a selective interaction in vitro between heme-hemopexin 
      and isolated rat hepatocytes has been demonstrated. This information is used as
      the basis for proposed models of the heme-binding site of hemopexin and of the
      interaction of heme-hemopexin with the parenchymal cells of the liver.
FAU - Morgan, W T
AU  - Morgan WT
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Finland
TA  - Ann Clin Res
JT  - Annals of clinical research
JID - 0220042
RN  - 0 (Porphyrins)
RN  - 42VZT0U6YR (Heme)
RN  - 9013-71-2 (Hemopexin)
SB  - IM
MH  - Animals
MH  - Biological Transport
MH  - Chemical Phenomena
MH  - Chemistry
MH  - Circular Dichroism
MH  - Heme/*metabolism
MH  - Hemopexin/analysis/*metabolism
MH  - Humans
MH  - Liver/*metabolism
MH  - Molecular Weight
MH  - Porphyrins/metabolism
MH  - *Protein Binding
MH  - Protein Conformation
MH  - Rats
MH  - Spectrum Analysis
EDAT- 1976/01/01 00:00
MHDA- 1976/01/01 00:01
CRDT- 1976/01/01 00:00
PHST- 1976/01/01 00:00 [pubmed]
PHST- 1976/01/01 00:01 [medline]
PHST- 1976/01/01 00:00 [entrez]
PST - ppublish
SO  - Ann Clin Res. 1976;8 Suppl 17:223-32.