PMID- 10082676
OWN - NLM
STAT- MEDLINE
DCOM- 19991217
LR  - 20171116
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 255
IP  - 1
DP  - 1999 Feb 5
TI  - Molecular cloning and expression of a second glucuronyltransferase involved in
      the biosynthesis of the HNK-1 carbohydrate epitope.
PG  - 182-7
AB  - A cDNA encoding a novel glucuronyltransferase was cloned from a rat brain cDNA
      library. The cDNA sequence contained an open reading frame encoding 324 amino
      acids, with type II transmembrane topology. The amino acid sequence revealed 49% 
      homology to rat GlcAT-P, a glucuronyltransferase involved in the biosynthesis of 
      the HNK-1 carbohydrate epitope of glycoproteins, [Terayama et al. (1997) Proc.
      Natl. Acad. Sci. USA 94, 6093-6098] and the highest sequence homology was found
      in the catalytic region. Northern blot analysis indicated that this newly cloned 
      glucuronyltransferase is expressed in the nervous system, consistent with the
      selective localization of the HNK-1 carbohydrate epitope in the nervous system.
      Transfection of this cDNA into COS-1 cells induced the expression of the HNK-1
      carbohydrate epitope on cell surfaces, and induced the morphological changes in
      these cells. These results indicated that this newly cloned cDNA is a second
      glucuronyltransferase involved in the biosynthesis of the HNK-1 carbohydrate
      epitope.
CI  - Copyright 1999 Academic Press.
FAU - Seiki, T
AU  - Seiki T
AD  - Department of Biological Chemistry and CREST (Core Research for Educational
      Science and Technology) Project, Japan Science and Technology Corporation, Kyoto,
      606-8501, Japan.
FAU - Oka, S
AU  - Oka S
FAU - Terayama, K
AU  - Terayama K
FAU - Imiya, K
AU  - Imiya K
FAU - Kawasaki, T
AU  - Kawasaki T
LA  - eng
SI  - GENBANK/AB010441
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (CD57 Antigens)
RN  - 0 (DNA, Complementary)
RN  - EC 2.4.1.17 (Glucuronosyltransferase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain
MH  - CD57 Antigens/*genetics
MH  - Cloning, Molecular
MH  - DNA, Complementary/*genetics/isolation & purification
MH  - Glucuronosyltransferase/*genetics
MH  - Molecular Sequence Data
MH  - Rats
EDAT- 1999/03/20 00:00
MHDA- 1999/03/20 00:01
CRDT- 1999/03/20 00:00
PHST- 1999/03/20 00:00 [pubmed]
PHST- 1999/03/20 00:01 [medline]
PHST- 1999/03/20 00:00 [entrez]
AID - S0006-291X(99)90151-1 [pii]
AID - 10.1006/bbrc.1999.0151 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Feb 5;255(1):182-7. doi: 10.1006/bbrc.1999.0151.