PMID- 10082570
OWN - NLM
STAT- MEDLINE
DCOM- 19990420
LR  - 20190508
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 4
DP  - 1999 Apr
TI  - A novel ubiquitin-specific protease, UBP43, cloned from leukemia fusion protein
      AML1-ETO-expressing mice, functions in hematopoietic cell differentiation.
PG  - 3029-38
AB  - Using PCR-coupled subtractive screening-representational difference analysis, we 
      have cloned a novel gene from AML1-ETO knockin mice. This gene is highly
      expressed in the yolk sac and fetal liver of the knockin mice. Nucleotide
      sequence analysis indicates that its cDNA contains an 1,107-bp open reading frame
      encoding a 368-amino-acid polypeptide. Further protein sequence and protein
      translation analysis shows that it belongs to a family of ubiquitin-specific
      proteases (UBP), and its molecular mass is 43 kDa. Therefore, we have named this 
      gene UBP43. Like other ubiquitin proteases, the UBP43 protein has
      deubiquitinating enzyme activity. Protein ubiquitination has been implicated in
      many important cellular events. In wild-type adult mice, UBP43 is highly
      expressed in the thymus and in peritoneal macrophages. Among nine different
      murine hematopoietic cell lines analyzed, UBP43 expression is detectable only in 
      cell lines related to the monocytic lineage. Furthermore, its expression is
      regulated during cytokine-induced monocytic cell differentiation. We have
      investigated its function in the hematopoietic myeloid cell line M1. UBP43 was
      introduced into M1 cells by retroviral gene transfer, and several high-expressing
      UBP43 clones were obtained for further study. Morphologic and cell surface marker
      examination of UBP43/M1 cells reveals that overexpression of UBP43 blocks
      cytokine-induced terminal differentiation of monocytic cells. These data suggest 
      that UBP43 plays an important role in hematopoiesis by modulating either the
      ubiquitin-dependent proteolytic pathway or the ubiquitination state of another
      regulatory factor(s) during myeloid cell differentiation.
FAU - Liu, L Q
AU  - Liu LQ
AD  - Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical
      School, Boston, Massachusetts 02115, USA.
FAU - Ilaria, R Jr
AU  - Ilaria R Jr
FAU - Kingsley, P D
AU  - Kingsley PD
FAU - Iwama, A
AU  - Iwama A
FAU - van Etten, R A
AU  - van Etten RA
FAU - Palis, J
AU  - Palis J
FAU - Zhang, D E
AU  - Zhang DE
LA  - eng
SI  - GENBANK/AF069502
GR  - CA/AI59589/CA/NCI NIH HHS/United States
GR  - R01 HL059484/HL/NHLBI NIH HHS/United States
GR  - HL 59484-01/HL/NHLBI NIH HHS/United States
GR  - P01 CA072009/CA/NCI NIH HHS/United States
GR  - CA/72009/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (AML1-ETO fusion protein, human)
RN  - 0 (Core Binding Factor Alpha 2 Subunit)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RUNX1 Translocation Partner 1 Protein)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Runx1 protein, mouse)
RN  - 0 (Transcription Factors)
RN  - 0 (Ubiquitins)
RN  - EC 3.4.- (Endopeptidases)
RN  - EC 3.4.19.- (Usp18 protein, mouse)
RN  - EC 3.4.19.12 (Ubiquitin Thiolesterase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Differentiation
MH  - Cloning, Molecular
MH  - Core Binding Factor Alpha 2 Subunit
MH  - *DNA-Binding Proteins
MH  - Endopeptidases/*genetics/metabolism
MH  - Hematopoiesis/*genetics
MH  - Hematopoietic Stem Cells/cytology
MH  - Leukemia, Experimental/*etiology/genetics
MH  - Leukemia, Myeloid, Acute/etiology/genetics
MH  - Macrophages/cytology
MH  - Mice
MH  - Mice, Knockout
MH  - Mice, Mutant Strains
MH  - Molecular Sequence Data
MH  - Monocytes/cytology
MH  - *Oncogene Proteins, Fusion
MH  - *Proto-Oncogene Proteins
MH  - RUNX1 Translocation Partner 1 Protein
MH  - Recombinant Fusion Proteins/genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Substrate Specificity
MH  - Transcription Factors/*genetics
MH  - Ubiquitin Thiolesterase
MH  - Ubiquitins/*metabolism
PMC - PMC84097
EDAT- 1999/03/19 03:04
MHDA- 2001/03/28 10:01
CRDT- 1999/03/19 03:04
PHST- 1999/03/19 03:04 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1999/03/19 03:04 [entrez]
AID - 10.1128/mcb.19.4.3029 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 Apr;19(4):3029-38. doi: 10.1128/mcb.19.4.3029.