PMID- 10082563
OWN - NLM
STAT- MEDLINE
DCOM- 19990420
LR  - 20191224
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 4
DP  - 1999 Apr
TI  - Fisp12/mouse connective tissue growth factor mediates endothelial cell adhesion
      and migration through integrin alphavbeta3, promotes endothelial cell survival,
      and induces angiogenesis in vivo.
PG  - 2958-66
AB  - Fisp12 was first identified as a secreted protein encoded by a growth
      factor-inducible immediate-early gene in mouse fibroblasts, whereas its human
      ortholog, CTGF (connective tissue growth factor), was identified as a mitogenic
      activity in conditioned media of human umbilical vein endothelial cells.
      Fisp12/CTGF is a member of a family of secreted proteins that includes CYR61,
      Nov, Elm-1, Cop-1/WISP-2, and WISP-3. Fisp12/CTGF has been shown to promote cell 
      adhesion and mitogenesis in both fibroblasts and endothelial cells and to
      stimulate cell migration in fibroblasts. These findings, together with the
      localization of Fisp12/CTGF in angiogenic tissues, as well as in atherosclerotic 
      plaques, suggest a possible role for Fisp12/CTGF in the regulation of vessel
      growth during development, wound healing, and vascular disease. In this study, we
      show that purified Fisp12 (mCTGF) protein promotes the adhesion of microvascular 
      endothelial cells through the integrin receptor alphavbeta3. Furthermore, Fisp12 
      stimulates the migration of microvascular endothelial cells in culture, also
      through an integrin-alphavbeta3-dependent mechanism. In addition, the presence of
      Fisp12 promotes endothelial cell survival when cells are plated on laminin and
      deprived of growth factors, a condition that otherwise induces apoptosis. In
      vivo, Fisp12 induces neovascularization in rat corneal micropocket implants.
      These results demonstrate that Fisp12 is a novel angiogenic inducer and suggest a
      direct role for Fisp12 in the adhesion, migration, and survival of endothelial
      cells during blood vessel growth. Taken together with the recent finding that the
      related protein CYR61 also induces angiogenesis, we suggest that Fisp12/mCTGF and
      CYR61 comprise prototypes of a new family of angiogenic regulators that function,
      at least in part, through integrin-alphavbeta3-dependent pathways.
FAU - Babic, A M
AU  - Babic AM
AD  - Department of Molecular Genetics, University of Illinois at Chicago College of
      Medicine, Chicago, Illinois 60607-7170, USA.
FAU - Chen, C C
AU  - Chen CC
FAU - Lau, L F
AU  - Lau LF
LA  - eng
GR  - R01 CA046565/CA/NCI NIH HHS/United States
GR  - CA46565/CA/NCI NIH HHS/United States
GR  - CA80080/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (CCN2 protein, human)
RN  - 0 (CCN2 protein, mouse)
RN  - 0 (CCN2 protein, rat)
RN  - 0 (CCN3 protein, human)
RN  - 0 (Ccn3 protein, mouse)
RN  - 0 (Growth Substances)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Nephroblastoma Overexpressed Protein)
RN  - 0 (Receptors, Vitronectin)
RN  - 139568-91-5 (Connective Tissue Growth Factor)
SB  - IM
MH  - Animals
MH  - Cell Adhesion
MH  - Cell Movement
MH  - Cell Survival
MH  - Connective Tissue Growth Factor
MH  - Cornea/blood supply
MH  - Endothelium, Vascular/cytology/*physiology
MH  - Growth Substances/*metabolism
MH  - Humans
MH  - Immediate-Early Proteins/*metabolism
MH  - *Intercellular Signaling Peptides and Proteins
MH  - *Neovascularization, Physiologic
MH  - Nephroblastoma Overexpressed Protein
MH  - Rats
MH  - Receptors, Vitronectin/*metabolism
PMC - PMC84090
EDAT- 1999/03/19 03:04
MHDA- 2000/03/18 09:00
CRDT- 1999/03/19 03:04
PHST- 1999/03/19 03:04 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 1999/03/19 03:04 [entrez]
AID - 10.1128/mcb.19.4.2958 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 Apr;19(4):2958-66. doi: 10.1128/mcb.19.4.2958.