PMID- 10080939
OWN - NLM
STAT- MEDLINE
DCOM- 19990419
LR  - 20191028
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 256
IP  - 3
DP  - 1999 Mar 24
TI  - ZF5, which is a Kruppel-type transcriptional repressor, requires the zinc finger 
      domain for self-association.
PG  - 573-8
AB  - ZF5, which we have cloned as a transcriptional repressor on the mouse c-myc
      promoter, has the POZ domain at the amino-terminus and the Kruppel-type zinc
      finger domain at the carboxy-terminus. In this report, we showed that ZF5 has two
      contradictory functions in transcription: activation of human immunodeficiency
      virus (HIV) promoter and repression of the HSV thymidine kinase (TK) promoter.
      The POZ domain contributed to the repressor activity, whereas the active function
      resulted from the DNA-binding ability of the zinc finger domain. We demonstrated 
      that the POZ domain has a function mediating homomeric protein-protein
      interaction and this interaction requires the zinc finger domain. Furthermore,
      the POZ domain decreased the DNA-binding activity of the zinc finger domain.
      These results can provide evidence indicating the important interaction between
      the POZ and zinc finger domains.
CI  - Copyright 1999 Academic Press.
FAU - Numoto, M
AU  - Numoto M
AD  - Research Institute for Radiation Biology and Medicine, Hiroshima University,
      1-2-3 Kasumi, Hiroshima, Minami-ku, 734, Japan. mnumoto@ipc.hiroshima-u.ac.jp
FAU - Yokoro, K
AU  - Yokoro K
FAU - Koshi, J
AU  - Koshi J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (ZBTB14 protien, human)
RN  - 0 (Zfp161 protein, mouse)
RN  - EC 2.7.1.21 (Thymidine Kinase)
SB  - IM
SB  - X
MH  - 3T3 Cells
MH  - Animals
MH  - Binding Sites
MH  - Blotting, Western
MH  - DNA-Binding Proteins/*chemistry/genetics/*metabolism
MH  - Dimerization
MH  - Gene Expression Regulation
MH  - HIV-1/genetics
MH  - Kruppel-Like Transcription Factors
MH  - Mice
MH  - Promoter Regions, Genetic/genetics
MH  - Protein Binding
MH  - Protein Biosynthesis
MH  - Recombinant Fusion Proteins/biosynthesis/metabolism
MH  - Repressor Proteins/chemistry/genetics/*metabolism
MH  - Sequence Deletion
MH  - Simplexvirus/genetics
MH  - Thymidine Kinase/genetics
MH  - Trans-Activators/chemistry/metabolism
MH  - Transcription Factors/*chemistry
MH  - Transfection
MH  - Zinc Fingers/genetics/*physiology
EDAT- 1999/03/19 00:00
MHDA- 1999/03/19 00:01
CRDT- 1999/03/19 00:00
PHST- 1999/03/19 00:00 [pubmed]
PHST- 1999/03/19 00:01 [medline]
PHST- 1999/03/19 00:00 [entrez]
AID - S0006-291X(99)90375-3 [pii]
AID - 10.1006/bbrc.1999.0375 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Mar 24;256(3):573-8. doi:
      10.1006/bbrc.1999.0375.