PMID- 10079200
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 256
IP  - 2
DP  - 1999 Mar 16
TI  - Bone morphogenetic protein-3b (BMP-3b) gene expression is correlated with
      differentiation in rat calvarial osteoblasts.
PG  - 419-24
AB  - BMP-3b (also called GDF-10) is a novel BMP-3-related protein recently discovered 
      in rat femur tissue. Gene expression of BMP-3b in osteoblastic cells and its
      regulation by prolonged culture, BMP-2 and transforming growth factor beta1
      (TGF-beta1) were examined. The BMP-3b gene was highly expressed in rat
      osteoblasts obtained from calvarial bones but not in the osteoblastic cell lines 
      (MC3T3-E1 and U2-OS). BMP-3b mRNA increased during osteoblastic differentiation
      in prolonged culture and was associated with increased alkaline phosphatase
      (ALPase) activity. When BMP-2, an enhancer of ALPase activity, was added to the
      primary osteoblast culture, BMP-3b mRNA increased 6.9-fold after 24 h. In
      contrast, TGF-beta1 treatment, which suppresses ALPase activity, rapidly and
      completely inhibited gene expression of BMP-3b. The regulation of BMP-3 mRNA
      differed from that of BMP-3b, even though both proteins share 81% identity. These
      findings indicate that BMP-3b gene expression is regulated by osteoblastic
      differentiation and BMP-3b functions in highly differentiated osteoblasts.
CI  - Copyright 1999 Academic Press.
FAU - Hino, J
AU  - Hino J
AD  - National Cardiovascular Center Research Institute, Osaka, Fujishirodai, Suita,
      565-8565, Japan.
FAU - Matsuo, H
AU  - Matsuo H
FAU - Kangawa, K
AU  - Kangawa K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Anthraquinones)
RN  - 0 (Bmp2 protein, rat)
RN  - 0 (Bmp3 protein, rat)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Bone Morphogenetic Protein 3)
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Gdf10 protein, rat)
RN  - 0 (Growth Differentiation Factor 10)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 5688UTC01R (Tretinoin)
RN  - 60MEW57T9G (alizarin)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Animals, Newborn
MH  - Anthraquinones
MH  - Bone Morphogenetic Protein 2
MH  - Bone Morphogenetic Protein 3
MH  - Bone Morphogenetic Proteins/*genetics/pharmacology
MH  - Calcification, Physiologic/drug effects
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cells, Cultured
MH  - Fibroblast Growth Factor 2/pharmacology
MH  - *Gene Expression Regulation/drug effects
MH  - Growth Differentiation Factor 10
MH  - Osteoblasts/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Skull/cytology/metabolism
MH  - Staining and Labeling
MH  - Time Factors
MH  - Transforming Growth Factor beta/pharmacology
MH  - Tretinoin/pharmacology
EDAT- 1999/03/18 00:00
MHDA- 1999/03/18 00:01
CRDT- 1999/03/18 00:00
PHST- 1999/03/18 00:00 [pubmed]
PHST- 1999/03/18 00:01 [medline]
PHST- 1999/03/18 00:00 [entrez]
AID - S0006-291X(99)90341-8 [pii]
AID - 10.1006/bbrc.1999.0341 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Mar 16;256(2):419-24. doi:
      10.1006/bbrc.1999.0341.