PMID- 10078553
OWN - NLM
STAT- MEDLINE
DCOM- 19990330
LR  - 20190515
IS  - 0012-1797 (Print)
IS  - 0012-1797 (Linking)
VI  - 48
IP  - 3
DP  - 1999 Mar
TI  - Molecular cloning of a pancreatic islet-specific glucose-6-phosphatase catalytic 
      subunit-related protein.
PG  - 531-42
AB  - A pancreatic islet-specific glucose-6-phosphatase-related protein (IGRP) was
      cloned using a subtractive cDNA expression cloning procedure from mouse
      insulinoma tissue. Two alternatively spliced variants that differed by the
      presence or absence of a 118-bp exon (exon IV) were detected in normal balb/c
      mice, diabetic ob/ob mice, and insulinoma tissue. The longer, 1901-bp full-length
      cDNA encoded a 355-amino acid protein (molecular weight 40,684) structurally
      related (50% overall identity) to the liver glucose-6-phosphatase and exhibited
      similar predicted transmembrane topology, conservation of catalytically important
      residues, and the presence of an endoplasmic reticulum retention signal. The
      shorter transcript encoded two possible open reading frames (ORFs), neither of
      which possessed His174, a residue thought to be the phosphoryl acceptor (Pan CJ, 
      Lei KJ, Annabi B, Hemrika W, Chou JY: Transmembrane topology of
      glucose-6-phosphatase. J Biol Chem 273:6144-6148, 1998). Northern blot and
      reverse transcription-polymerase chain reaction analysis showed that the mRNA was
      highly expressed in pancreatic islets and expressed more in beta-cell lines than 
      in an alpha-cell line. It was notably absent in tissues and cell lines of
      non-islet neuroendocrine origin, and no other major tissue source of the mRNA was
      found. During development, it was expressed in parallel with insulin mRNA. The
      mRNA was efficiently translated and glycosylated in an in vitro
      translation/membrane translocation system and readily transcribed into COS 1,
      HIT, and CHO cells using cytomegalovirus or Rous sarcoma virus promoters. Whereas
      the liver glucose-6-phosphatase showed activity in these transfection systems,
      the IGRP failed to show glucose phosphotransferase or phosphatase activity with
      p-nitrophenol phosphate, inorganic pyrophosphate, or a range of sugar phosphates 
      hydrolyzed by the liver enzyme. While the metabolic function of the enzyme is not
      resolved, its remarkable tissue-specific expression warrants further
      investigation, as does its transcriptional regulation in conditions where glucose
      responsiveness of the pancreatic islet is altered.
FAU - Arden, S D
AU  - Arden SD
AD  - Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's
      Hospital, UK.
FAU - Zahn, T
AU  - Zahn T
FAU - Steegers, S
AU  - Steegers S
FAU - Webb, S
AU  - Webb S
FAU - Bergman, B
AU  - Bergman B
FAU - O'Brien, R M
AU  - O'Brien RM
FAU - Hutton, J C
AU  - Hutton JC
LA  - eng
SI  - GENBANK/Z47787
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Diabetes
JT  - Diabetes
JID - 0372763
RN  - 0 (Proteins)
RN  - EC 3.1.3.9 (Glucose-6-Phosphatase)
RN  - EC 3.1.3.9. (G6PC2 protein, human)
RN  - EC 3.1.3.9. (G6pc2 protein, mouse)
SB  - AIM
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Membrane/metabolism
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - Dogs
MH  - Exons
MH  - Fishes
MH  - Genetic Variation
MH  - Glucose-6-Phosphatase/chemistry/genetics
MH  - Humans
MH  - Insulinoma/genetics/metabolism
MH  - Islets of Langerhans/*metabolism
MH  - Liver/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Obese
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Pancreatic Neoplasms/genetics/metabolism
MH  - Protein Structure, Secondary
MH  - Proteins/chemistry/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
EDAT- 1999/03/17 00:00
MHDA- 1999/03/17 00:01
CRDT- 1999/03/17 00:00
PHST- 1999/03/17 00:00 [pubmed]
PHST- 1999/03/17 00:01 [medline]
PHST- 1999/03/17 00:00 [entrez]
AID - 10.2337/diabetes.48.3.531 [doi]
PST - ppublish
SO  - Diabetes. 1999 Mar;48(3):531-42. doi: 10.2337/diabetes.48.3.531.