PMID- 10078534
OWN - NLM
STAT- MEDLINE
DCOM- 19990325
LR  - 20131121
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 398
IP  - 6722
DP  - 1999 Mar 4
TI  - DREAM is a Ca2+-regulated transcriptional repressor.
PG  - 80-4
AB  - Fluxes in amounts of intracellular calcium ions are important determinants of
      gene expression. So far, Ca2+-regulated kinases and phosphatases have been
      implicated in changing the phosphorylation status of key transcription factors
      and thereby modulating their function. In addition, direct effectors of
      Ca2+-induced gene expression have been suggested to exist in the nucleus,
      although no such effectors have been identified yet. Expression of the human
      prodynorphin gene, which is involved in memory acquisition and pain, is regulated
      through its downstream regulatory element (DRE) sequence, which acts as a
      location-dependent gene silencer. Here we isolate a new transcriptional
      repressor, DRE-antagonist modulator (DREAM), which specifically binds to the DRE.
      DREAM contains four Ca2+-binding domains of the EF-hand type. Upon stimulation by
      Ca2+, DREAM's ability to bind to the DRE and its repressor function are
      prevented. Mutation of the EF-hands abolishes the response of DREAM to Ca2+. In
      addition to the prodynorphin promoter, DREAM represses transcription from the
      early response gene c-fos. Thus, DREAM represents the first known Ca2+-binding
      protein to function as a DNA-binding transcriptional regulator.
FAU - Carrion, A M
AU  - Carrion AM
AD  - Instituto de Neurobiologia S. Ramon y Cajal, CSIC, Madrid, Spain.
FAU - Link, W A
AU  - Link WA
FAU - Ledo, F
AU  - Ledo F
FAU - Mellstrom, B
AU  - Mellstrom B
FAU - Naranjo, J R
AU  - Naranjo JR
LA  - eng
SI  - GENBANK/AJ131730
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Enkephalins)
RN  - 0 (KCNIP3 protein, human)
RN  - 0 (Kv Channel-Interacting Proteins)
RN  - 0 (Protein Precursors)
RN  - 0 (Repressor Proteins)
RN  - 9007-49-2 (DNA)
RN  - 93443-35-7 (preproenkephalin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
CIN - Nature. 1999 Mar 4;398(6722):29-30. PMID: 10078526
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Calcium/metabolism
MH  - Calcium-Binding Proteins/chemistry/genetics/*physiology
MH  - Cell Line
MH  - DNA/metabolism
MH  - DNA-Binding Proteins/chemistry/genetics/physiology
MH  - Enkephalins/genetics
MH  - *Gene Expression Regulation
MH  - Genes, fos
MH  - Humans
MH  - Kv Channel-Interacting Proteins
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - Protein Precursors/genetics
MH  - Repressor Proteins/chemistry/genetics/*physiology
MH  - Sequence Homology, Amino Acid
MH  - Transcription, Genetic
MH  - Transfection
EDAT- 1999/03/17 03:05
MHDA- 2001/03/23 10:01
CRDT- 1999/03/17 03:05
PHST- 1999/03/17 03:05 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/03/17 03:05 [entrez]
AID - 10.1038/18044 [doi]
PST - ppublish
SO  - Nature. 1999 Mar 4;398(6722):80-4. doi: 10.1038/18044.