PMID- 10078207
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20190915
IS  - 1097-2765 (Print)
IS  - 1097-2765 (Linking)
VI  - 3
IP  - 2
DP  - 1999 Feb
TI  - Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits.
PG  - 247-53
AB  - Protein complexes of the SWI/SNF family remodel nucleosome structure in an
      ATP-dependent manner. Each complex contains between 8 and 15 subunits, several of
      which are highly conserved between yeast, Drosophila, and humans. We have
      reconstituted an ATP-dependent chromatin remodeling complex using a subset of
      conserved subunits. Unexpectedly, both BRG1 and hBRM, the ATPase subunits of
      human SWI/SNF complexes, are capable of remodeling mono-nucleosomes and
      nucleosomal arrays as purified proteins. The addition of INI1, BAF155, and BAF170
      to BRG1 increases remodeling activity to a level comparable to that of the whole 
      hSWI/SNF complex. These data define the functional core of the hSWI/SNF complex.
FAU - Phelan, M L
AU  - Phelan ML
AD  - Department of Molecular Biology, Massachusetts General Hospital, Boston 02114,
      USA.
FAU - Sif, S
AU  - Sif S
FAU - Narlikar, G J
AU  - Narlikar GJ
FAU - Kingston, R E
AU  - Kingston RE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell
JT  - Molecular cell
JID - 9802571
RN  - 0 (ACTL6A protein, human)
RN  - 0 (Actins)
RN  - 0 (Chromatin)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nucleosomes)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Ribonucleoprotein, U1 Small Nuclear)
RN  - 0 (SMARCA2 protein, human)
RN  - 0 (SMARCC1 protein, human)
RN  - 0 (SMARCC2 protein, human)
RN  - 0 (SMARCD1 protein, human)
RN  - 0 (Transcription Factors)
RN  - 0 (snf protein, Drosophila)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 3.6.1.- (SMARCA4 protein, human)
RN  - EC 3.6.4.- (DNA Helicases)
SB  - IM
MH  - Actins/*chemistry/physiology
MH  - Adenosine Triphosphate/physiology
MH  - Cell-Free System
MH  - Chromatin/metabolism/*ultrastructure
MH  - *Chromosomal Proteins, Non-Histone
MH  - DNA Helicases
MH  - DNA-Binding Proteins/*chemistry/physiology
MH  - *Drosophila Proteins
MH  - Evolution, Molecular
MH  - Humans
MH  - Macromolecular Substances
MH  - Nuclear Proteins/*chemistry/physiology
MH  - Nucleosomes/metabolism/*ultrastructure
MH  - *RNA-Binding Proteins
MH  - Recombinant Fusion Proteins/chemistry
MH  - Ribonucleoprotein, U1 Small Nuclear/*chemistry/physiology
MH  - Transcription Factors/*chemistry/physiology
EDAT- 1999/03/17 00:00
MHDA- 1999/03/17 00:01
CRDT- 1999/03/17 00:00
PHST- 1999/03/17 00:00 [pubmed]
PHST- 1999/03/17 00:01 [medline]
PHST- 1999/03/17 00:00 [entrez]
AID - S1097-2765(00)80315-9 [pii]
AID - 10.1016/s1097-2765(00)80315-9 [doi]
PST - ppublish
SO  - Mol Cell. 1999 Feb;3(2):247-53. doi: 10.1016/s1097-2765(00)80315-9.