PMID- 10078204
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20190915
IS  - 1097-2765 (Print)
IS  - 1097-2765 (Linking)
VI  - 3
IP  - 2
DP  - 1999 Feb
TI  - Use of altered specificity mutants to probe a specific protein-protein
      interaction in differentiation: the GATA-1:FOG complex.
PG  - 219-28
AB  - GATA-1 and FOG (Friend of GATA-1) are each essential for erythroid and
      megakaryocyte development. FOG, a zinc finger protein, interacts with the amino
      (N) finger of GATA-1 and cooperates with GATA-1 to promote differentiation. To
      determine whether this interaction is critical for GATA-1 action, we selected
      GATA-1 mutants in yeast that fail to interact with FOG but retain normal DNA
      binding, as well a compensatory FOG mutant that restores interaction. These novel
      GATA-1 mutants do not promote erythroid differentiation of GATA-1- erythroid
      cells. Differentiation is rescued by the second-site FOG mutant. Thus,
      interaction of FOG with GATA-1 is essential for the function of GATA-1 in
      erythroid differentiation. These findings provide a paradigm for dissecting
      protein-protein associations involved in mammalian development.
FAU - Crispino, J D
AU  - Crispino JD
AD  - Division of Hematology-Oncology, Children's Hospital, Boston, Massachusetts
      02115, USA.
FAU - Lodish, M B
AU  - Lodish MB
FAU - MacKay, J P
AU  - MacKay JP
FAU - Orkin, S H
AU  - Orkin SH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell
JT  - Molecular cell
JID - 9802571
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Erythroid-Specific DNA-Binding Factors)
RN  - 0 (GATA1 Transcription Factor)
RN  - 0 (Gata1 protein, mouse)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Zfpm1 protein, mouse)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carrier Proteins/genetics/*metabolism
MH  - Cell Differentiation
MH  - DNA/metabolism
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Erythroid Precursor Cells/metabolism
MH  - Erythroid-Specific DNA-Binding Factors
MH  - Erythropoiesis/*physiology
MH  - GATA1 Transcription Factor
MH  - Gene Expression Regulation
MH  - Genetic Complementation Test
MH  - Macromolecular Substances
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Saccharomyces cerevisiae/genetics
MH  - Structure-Activity Relationship
MH  - Transcription Factors/genetics/*metabolism
MH  - Transfection
MH  - Zinc Fingers
EDAT- 1999/03/17 00:00
MHDA- 1999/03/17 00:01
CRDT- 1999/03/17 00:00
PHST- 1999/03/17 00:00 [pubmed]
PHST- 1999/03/17 00:01 [medline]
PHST- 1999/03/17 00:00 [entrez]
AID - S1097-2765(00)80312-3 [pii]
AID - 10.1016/s1097-2765(00)80312-3 [doi]
PST - ppublish
SO  - Mol Cell. 1999 Feb;3(2):219-28. doi: 10.1016/s1097-2765(00)80312-3.