PMID- 10077700
OWN - NLM
STAT- MEDLINE
DCOM- 19990714
LR  - 20171101
IS  - 0378-5866 (Print)
IS  - 0378-5866 (Linking)
VI  - 21
IP  - 1
DP  - 1999
TI  - Reduced levels of a specific myelin-associated oligodendrocytic basic protein
      isoform in shiverer myelin.
PG  - 36-42
AB  - Myelin-associated oligodendrocytic basic protein (MOBP) and myelin basic protein 
      (MBP) share many structural similarities. MOBP is synthesised by mature
      oligodendrocytes and localised at the major dense line (MDL), suggesting a role
      in the myelin compaction process. The shiverer mouse, a deletion mutant of the
      myelin basic protein (Mbp) gene, has poorly compacted myelin with essentially no 
      MDL. In this study we compare the developmental expression of the Mobp gene in
      wild-type and shiverer mice. The significant finding is that one of the two
      abundant MOBP isoforms, the approximately 20-kD species, is poorly incorporated
      into shiverer myelin. The absence is specific to shiverer and is not a feature of
      dysmyelinating mutants with an abnormal intraperiod line. Our data suggest that
      incorporation of this MOBP isoform into shiverer myelin may be influenced by the 
      presence of MBP or be a consequence of a disrupted MDL.
FAU - Montague, P
AU  - Montague P
AD  - Applied Neurobiology Group, Department of Veterinary Clinical Studies, University
      of Glasgow, Bearsden, UK.P.Montague@vet.gla.ac.uk
FAU - Kirkham, D
AU  - Kirkham D
FAU - McCallion, A S
AU  - McCallion AS
FAU - Davies, R W
AU  - Davies RW
FAU - Kennedy, P G
AU  - Kennedy PG
FAU - Klugmann, M
AU  - Klugmann M
FAU - Nave, K
AU  - Nave K
FAU - Griffiths, I R
AU  - Griffiths IR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Dev Neurosci
JT  - Developmental neuroscience
JID - 7809375
RN  - 0 (Mobp protein, mouse)
RN  - 0 (Mog protein, mouse)
RN  - 0 (Myelin Proteins)
RN  - 0 (Myelin-Associated Glycoprotein)
RN  - 0 (Myelin-Oligodendrocyte Glycoprotein)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Aging
MH  - Animals
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Brain/metabolism
MH  - Fluorescent Antibody Technique
MH  - Gene Expression
MH  - In Situ Hybridization
MH  - Mice
MH  - Mice, Jimpy
MH  - Mice, Neurologic Mutants
MH  - Myelin Proteins
MH  - Myelin Sheath/*chemistry/*genetics
MH  - Myelin-Associated Glycoprotein/analysis/*metabolism
MH  - Myelin-Oligodendrocyte Glycoprotein
MH  - Protein Isoforms/analysis/metabolism
MH  - RNA, Messenger/metabolism
MH  - Spinal Cord/metabolism
EDAT- 1999/03/17 03:04
MHDA- 2000/08/16 11:00
CRDT- 1999/03/17 03:04
PHST- 1999/03/17 03:04 [pubmed]
PHST- 2000/08/16 11:00 [medline]
PHST- 1999/03/17 03:04 [entrez]
AID - 17364 [pii]
AID - 10.1159/000017364 [doi]
PST - ppublish
SO  - Dev Neurosci. 1999;21(1):36-42. doi: 10.1159/000017364.