PMID- 10077664
OWN - NLM
STAT- MEDLINE
DCOM- 19990520
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 6
DP  - 1999 Mar 16
TI  - Cortical bitufted, horizontal, and Martinotti cells preferentially express and
      secrete reelin into perineuronal nets, nonsynaptically modulating gene
      expression.
PG  - 3217-22
AB  - Reelin (Reln) is a protein with some structural analogies with other
      extracellular matrix proteins that functions in the regulation of neuronal
      migration during the development of cortical laminated structures. In the cortex 
      of adult animals, Reln is expressed primarily in gamma-aminobutyric acid
      (GABA)ergic neurons and is secreted into perineuronal nets. However, only 50-60% 
      of GABAergic interneurons express Reln. We have characterized this subpopulation 
      of cortical GABAergic neurons that expresses Reln by using two strategies: (i) a 
      double immunolabeling procedure to determine the colocalization of Reln with
      neuropeptides and Ca2+-binding proteins and (ii) a combination of Golgi staining 
      and Reln immunolabeling to determine the morphology of the rat cortical cells
      that store Reln. Many interneurons that express Neuropeptide Y (NPY) or
      somatostatin (but none of those that express parvalbumin) are
      Reln-immunopositive. A small population of calbindin-positive interneurons and
      very few calretinin-positive cells express Reln immunopositivity. Golgi staining 
      revealed that layer I horizontal cells, layer II-V bitufted neurons, and some
      deep cortical layer Martinotti cells express Reln. Basket and chandelier cells
      are often immunopositive to parvalbumin, but never to Reln. Although Reln is
      secreted by GABAergic neurons, its target are not the GABA receptors, but rather 
      may be extrasynaptically located in perineuronal nets and concerned with the
      modulation of neuronal plasticity. Dab1, the target adapter protein that
      presumably mediates transcription regulation via the extrasynaptic actions of
      Reln, is expressed predominantly in pyramidal neurons, but it can also be
      detected in a small population of GABAergic neurons that are neither horizontal
      nor bitufted neurons.
FAU - Pesold, C
AU  - Pesold C
AD  - Psychiatric Institute, Department of Psychiatry, College of Medicine, University 
      of Illinois at Chicago, 1601 West Taylor Street, M/C 912, Chicago IL, 60612, USA.
      cpesold@psych.uni.edu
FAU - Liu, W S
AU  - Liu WS
FAU - Guidotti, A
AU  - Guidotti A
FAU - Costa, E
AU  - Costa E
FAU - Caruncho, H J
AU  - Caruncho HJ
LA  - eng
GR  - MH 49486-07/MH/NIMH NIH HHS/United States
GR  - MH 56500-01/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Cell Adhesion Molecules, Neuronal)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.- (reelin protein)
SB  - IM
MH  - Animals
MH  - Cell Adhesion Molecules, Neuronal/*biosynthesis/physiology
MH  - Cerebral Cortex/cytology/*physiology
MH  - Extracellular Matrix/*physiology
MH  - Extracellular Matrix Proteins/*biosynthesis/physiology
MH  - Gene Expression Regulation/*physiology
MH  - Nerve Tissue Proteins/biosynthesis/metabolism
MH  - Rats
MH  - Rats, Inbred F344
MH  - Serine Endopeptidases
MH  - Synapses/physiology
MH  - gamma-Aminobutyric Acid/physiology
PMC - PMC15922
EDAT- 1999/03/17 00:00
MHDA- 1999/03/17 00:01
CRDT- 1999/03/17 00:00
PHST- 1999/03/17 00:00 [pubmed]
PHST- 1999/03/17 00:01 [medline]
PHST- 1999/03/17 00:00 [entrez]
AID - 10.1073/pnas.96.6.3217 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):3217-22. doi: 10.1073/pnas.96.6.3217.