PMID- 10077619
OWN - NLM
STAT- MEDLINE
DCOM- 19990520
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 6
DP  - 1999 Mar 16
TI  - Organization of human and mouse skeletal myosin heavy chain gene clusters is
      highly conserved.
PG  - 2958-63
AB  - Myosin heavy chains (MyHCs) are highly conserved ubiquitous actin-based motor
      proteins that drive a wide range of motile processes in eukaryotic cells. MyHC
      isoforms expressed in skeletal muscles are encoded by a multigene family that is 
      clustered on syntenic regions of human and mouse chromosomes 17 and 11,
      respectively. In an effort to gain a better understanding of the genomic
      organization of the skeletal MyHC genes and its effects on the regulation,
      function, and molecular genetics of this multigene family, we have constructed
      high-resolution physical maps of both human and mouse loci using PCR-based marker
      content mapping of P1-artificial chromosome clones. Genes encoding six MyHC
      isoforms have been mapped with respect to their linear order and transcriptional 
      orientations within a 350-kb region in both human and mouse. These maps reveal
      that the order, transcriptional orientation, and relative intergenic distances of
      these genes are remarkably conserved between these species. Unlike many clustered
      gene families, this order does not reflect the known temporal expression patterns
      of these genes. However, the conservation of gene organization since the
      estimated divergence of these species (approximately 75-110 million years ago)
      suggests that the physical organization of these genes may be significant for
      their regulation and function.
FAU - Weiss, A
AU  - Weiss A
AD  - Department of Microbiology and Immunology, Albert Einstein College of Medicine,
      1300 Morris Park Avenue, Bronx, NY 10461, USA.
FAU - McDonough, D
AU  - McDonough D
FAU - Wertman, B
AU  - Wertman B
FAU - Acakpo-Satchivi, L
AU  - Acakpo-Satchivi L
FAU - Montgomery, K
AU  - Montgomery K
FAU - Kucherlapati, R
AU  - Kucherlapati R
FAU - Leinwand, L
AU  - Leinwand L
FAU - Krauter, K
AU  - Krauter K
LA  - eng
SI  - GENBANK/AF111782
SI  - GENBANK/AF111783
SI  - GENBANK/AF111784
SI  - GENBANK/AF111785
GR  - R01 GM029090/GM/NIGMS NIH HHS/United States
GR  - 5T32GM07135/GM/NIGMS NIH HHS/United States
GR  - P60 DA11015-02/DA/NIDA NIH HHS/United States
GR  - GM29090/GM/NIGMS NIH HHS/United States
GR  - T32 GM007135/GM/NIGMS NIH HHS/United States
GR  - P60 DA011015/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - EC 3.6.4.1 (Myosin Heavy Chains)
SB  - IM
MH  - Animals
MH  - Chromosome Mapping
MH  - *Conserved Sequence
MH  - Humans
MH  - Mice
MH  - *Multigene Family
MH  - Muscle, Skeletal/metabolism
MH  - Myosin Heavy Chains/*genetics
PMC - PMC15877
EDAT- 1999/03/17 00:00
MHDA- 1999/03/17 00:01
CRDT- 1999/03/17 00:00
PHST- 1999/03/17 00:00 [pubmed]
PHST- 1999/03/17 00:01 [medline]
PHST- 1999/03/17 00:00 [entrez]
AID - 10.1073/pnas.96.6.2958 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2958-63. doi: 10.1073/pnas.96.6.2958.