PMID- 10077598
OWN - NLM
STAT- MEDLINE
DCOM- 19990520
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 6
DP  - 1999 Mar 16
TI  - Identification of a putative effector protein for rab11 that participates in
      transferrin recycling.
PG  - 2840-5
AB  - We have identified and cloned the cDNA for a 912-aa protein, rab11BP, that
      interacts with the GTP-containing active form of rab11, a GTP-binding protein
      that plays a critical role in receptor recycling. Although rab11BP is primarily
      cytosolic, a significant fraction colocalizes with rab11 in endosomal membranes
      of both the sorting and recycling subcompartments. In vitro binding of rab11 to
      native rab11BP requires partial denaturation of the latter to expose an internal 
      binding site located between residues 334 and 504 that is apparently masked by
      the C-terminal portion of the protein, which includes six repeats known as WD40
      domains. Within the cell, rab11BP must undergo a conformational change in which
      the rab11-binding site becomes exposed, because when coexpressed with rab11 in
      transfected cells the two proteins formed abundant complexes in association with 
      membranes. Furthermore, although overexpression of rab11BP did not affect
      transferrin recycling, overexpression of a truncated form of the protein,
      rab11BP(1-504), that includes the rab11-binding site but lacks the WD40 domains
      inhibited recycling as strongly as does a dominant negative rab11 mutant protein 
      that does not bind GTP. Strikingly, the inhibition caused by the truncated
      rab11BP was prevented completely when the cells also expressed a C-terminally
      deleted, nonprenylatable form of rab11 that, by itself, has no effect on
      recycling. We propose that rab11BP is an effector for rab11, whose association
      with this GTP-binding protein is dependent on the action of another
      membrane-associated factor that promotes the unmasking of the rab11-binding site 
      in rab11BP.
FAU - Zeng, J
AU  - Zeng J
AD  - Department of Cell Biology and Kaplan Cancer Center, New York University School
      of Medicine, 550 First Avenue, New York, NY 10016-6497, USA.
FAU - Ren, M
AU  - Ren M
FAU - Gravotta, D
AU  - Gravotta D
FAU - De Lemos-Chiarandini, C
AU  - De Lemos-Chiarandini C
FAU - Lui, M
AU  - Lui M
FAU - Erdjument-Bromage, H
AU  - Erdjument-Bromage H
FAU - Tempst, P
AU  - Tempst P
FAU - Xu, G
AU  - Xu G
FAU - Shen, T H
AU  - Shen TH
FAU - Morimoto, T
AU  - Morimoto T
FAU - Adesnik, M
AU  - Adesnik M
FAU - Sabatini, D D
AU  - Sabatini DD
LA  - eng
SI  - GENBANK/AF117897
GR  - R01 GM043583/GM/NIGMS NIH HHS/United States
GR  - GM43583/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA, Complementary)
RN  - 0 (Transferrin)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.1.- (rab11 protein)
RN  - EC 3.6.5.2 (rab GTP-Binding Proteins)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cloning, Molecular
MH  - DNA, Complementary/analysis/genetics
MH  - GTP-Binding Proteins/*genetics/*metabolism
MH  - Gene Expression Regulation
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Transferrin/*metabolism
MH  - *rab GTP-Binding Proteins
PMC - PMC15856
EDAT- 1999/03/17 00:00
MHDA- 1999/03/17 00:01
CRDT- 1999/03/17 00:00
PHST- 1999/03/17 00:00 [pubmed]
PHST- 1999/03/17 00:01 [medline]
PHST- 1999/03/17 00:00 [entrez]
AID - 10.1073/pnas.96.6.2840 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2840-5. doi: 10.1073/pnas.96.6.2840.