PMID- 10077576
OWN - NLM
STAT- MEDLINE
DCOM- 19990520
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 6
DP  - 1999 Mar 16
TI  - Characterization of Sam68-like mammalian proteins SLM-1 and SLM-2: SLM-1 is a Src
      substrate during mitosis.
PG  - 2710-5
AB  - Sam68, the 68-kDa Src substrate associated during mitosis, is an RNA-binding
      protein with signaling properties that contains a GSG (GRP33, Sam68, GLD-1)
      domain. Here we report the cloning of two Sam68-like-mammalian proteins, SLM-1
      and SLM-2. These proteins have an approximately 70% sequence identity with Sam68 
      in their GSG domain. SLM-1 and SLM-2 have the characteristic Sam68 SH2 and SH3
      domain binding sites. SLM-1 is an RNA-binding protein that is tyrosine
      phosphorylated by Src during mitosis. SLM-1 bound the SH2 and SH3 domains of
      p59(fyn), Grb-2, phospholipase Cgamma-1 (PLCgamma-1), and/or p120(rasGAP),
      suggesting it may function as a multifunctional adapter protein for Src during
      mitosis. SLM-2 is an RNA-binding protein that is not tyrosine phosphorylated by
      Src or p59(fyn). Moreover, SLM-2 did not associate with the SH3 domains of
      p59(fyn), Grb-2, PLCgamma-1, or p120(rasGAP), suggesting that SLM-2 may not
      function as an adapter protein for these proteins. The identification of SLM-1
      and SLM-2 demonstrates the presence of a Sam68/SLM family whose members have the 
      potential to link signaling pathways with RNA metabolism.
FAU - Di Fruscio, M
AU  - Di Fruscio M
AD  - Terry Fox Molecular Oncology Group, Lady Davis Institute for Medical Research,
      Sir Mortimer B. Davis Jewish General Hospital, Department of Oncology, McGill
      University, Montreal, PQ H3T 1E2, Canada.
FAU - Chen, T
AU  - Chen T
FAU - Richard, S
AU  - Richard S
LA  - eng
SI  - GENBANK/AF098796
SI  - GENBANK/AF099092
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (KHDRBS1 protein, human)
RN  - 0 (KHDRBS2 protein, human)
RN  - 0 (KHDRBS3 protein, human)
RN  - 0 (Khdrbs1 protein, mouse)
RN  - 0 (Khdrbs2 protein, mouse)
RN  - 0 (Khdrbs3 protein, mouse)
RN  - 0 (RNA-Binding Proteins)
RN  - EC 2.7.10.2 (src-Family Kinases)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - DNA-Binding Proteins
MH  - HeLa Cells
MH  - Humans
MH  - Mice
MH  - Mitosis/*genetics
MH  - Molecular Sequence Data
MH  - RNA-Binding Proteins/*genetics/metabolism
MH  - Sequence Alignment
MH  - Substrate Specificity
MH  - src-Family Kinases/*genetics/metabolism
PMC - PMC15834
EDAT- 1999/03/17 00:00
MHDA- 1999/03/17 00:01
CRDT- 1999/03/17 00:00
PHST- 1999/03/17 00:00 [pubmed]
PHST- 1999/03/17 00:01 [medline]
PHST- 1999/03/17 00:00 [entrez]
AID - 10.1073/pnas.96.6.2710 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2710-5. doi: 10.1073/pnas.96.6.2710.