PMID- 10075978
OWN - NLM
STAT- MEDLINE
DCOM- 19990527
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 0022-1007 (Linking)
VI  - 189
IP  - 6
DP  - 1999 Mar 15
TI  - A novel gene coding for a Fas apoptosis inhibitory molecule (FAIM) isolated from 
      inducibly Fas-resistant B lymphocytes.
PG  - 949-56
AB  - The sensitivity of primary splenic B cells to Fas-mediated apoptosis is modulated
      in a receptor-specific fashion. Here we used a differential display strategy to
      detect cDNAs present in B cells rendered Fas resistant but absent in those
      rendered Fas sensitive. This led to the cloning and characterization of a novel
      1.2-kb gene that encodes a Fas apoptosis inhibitory molecule (FAIM).
      faim-transfected BAL-17 B lymphoma cells were less sensitive by half or more to
      Fas-mediated apoptosis than were vector-transfected controls, using Fas
      ligand-bearing T cells or a cytotoxic anti-Fas antibody to trigger Fas, and this 
      was associated with inhibition of Fas- induced poly-ADP ribose polymerase (PARP) 
      cleavage. In primary B cells, the time course of faim mRNA and FAIM protein
      expression correlated with the induction of Fas resistance by surface (s)Ig
      engagement. Thus, FAIM is an inducible effector molecule that mediates Fas
      resistance produced by sIg engagement in B cells. However, faim is broadly
      expressed in various tissues and the faim sequence is highly conserved
      evolutionarily, suggesting that its role extends beyond lymphocyte homeostasis.
      As FAIM has no significant regions of homology to other gene products that
      modulate Fas killing, it appears to represent a distinct, new class of
      antiapoptotic protein.
FAU - Schneider, T J
AU  - Schneider TJ
AD  - Department of Microbiology, Boston University Medical Center, Boston,
      Massachusetts 02118, USA.
FAU - Fischer, G M
AU  - Fischer GM
FAU - Donohoe, T J
AU  - Donohoe TJ
FAU - Colarusso, T P
AU  - Colarusso TP
FAU - Rothstein, T L
AU  - Rothstein TL
LA  - eng
SI  - GENBANK/AF130367
GR  - R01 AI040181/AI/NIAID NIH HHS/United States
GR  - R21 AI040181/AI/NIAID NIH HHS/United States
GR  - AI40181/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (DNA, Complementary)
RN  - 0 (fas Receptor)
RN  - EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - B-Lymphocytes/*metabolism
MH  - Conserved Sequence
MH  - DNA, Complementary/*isolation & purification
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Poly(ADP-ribose) Polymerases/metabolism
MH  - fas Receptor/*physiology
PMC - PMC2193037
EDAT- 1999/03/17 00:00
MHDA- 1999/03/17 00:01
CRDT- 1999/03/17 00:00
PHST- 1999/03/17 00:00 [pubmed]
PHST- 1999/03/17 00:01 [medline]
PHST- 1999/03/17 00:00 [entrez]
AID - 10.1084/jem.189.6.949 [doi]
PST - ppublish
SO  - J Exp Med. 1999 Mar 15;189(6):949-56. doi: 10.1084/jem.189.6.949.