PMID- 10075920
OWN - NLM
STAT- MEDLINE
DCOM- 19990525
LR  - 20181113
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 6
DP  - 1999 Mar 15
TI  - EFA6, a sec7 domain-containing exchange factor for ARF6, coordinates membrane
      recycling and actin cytoskeleton organization.
PG  - 1480-91
AB  - We have identified a human cDNA encoding a novel protein, exchange factor for
      ARF6 (EFA6), which contains Sec7 and pleckstrin homology domains. EFA6 promotes
      efficient guanine nucleotide exchange on ARF6 and is distinct from the ARNO
      family of ARF1 exchange factors. The protein localizes to a dense matrix on the
      cytoplasmic face of plasma membrane invaginations, induced on its expression. We 
      show that EFA6 regulates endosomal membrane recycling and promotes the
      redistribution of transferrin receptors to the cell surface. Furthermore,
      expression of EFA6 induces actin-based membrane ruffles that are inhibited by
      co-expression of dominant-inhibitory mutant forms of ARF6 or Rac1. Our results
      demonstrate that by catalyzing nucleotide exchange on ARF6 at the plasma membrane
      and by regulating Rac1 activation, EFA6 coordinates endocytosis with cytoskeletal
      rearrangements.
FAU - Franco, M
AU  - Franco M
AD  - Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, Case 906, 13288 Marseille
      Cedex 9, France.
FAU - Peters, P J
AU  - Peters PJ
FAU - Boretto, J
AU  - Boretto J
FAU - van Donselaar, E
AU  - van Donselaar E
FAU - Neri, A
AU  - Neri A
FAU - D'Souza-Schorey, C
AU  - D'Souza-Schorey C
FAU - Chavrier, P
AU  - Chavrier P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Actins)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Fungal Proteins)
RN  - 0 (Guanine Nucleotide Exchange Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (PSD protein, human)
RN  - 0 (Peptide Elongation Factors)
RN  - 0 (Receptors, Transferrin)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Sec7 guanine nucleotide exchange factors)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (ADP-Ribosylation Factor 1)
RN  - EC 3.6.5.2 (ADP-Ribosylation Factors)
SB  - IM
MH  - ADP-Ribosylation Factor 1
MH  - ADP-Ribosylation Factors
MH  - Actins/chemistry/*metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - CHO Cells
MH  - Carrier Proteins/chemistry/metabolism
MH  - Cricetinae
MH  - Cytoskeleton/*ultrastructure
MH  - DNA, Complementary
MH  - Fungal Proteins/*chemistry
MH  - GTP-Binding Proteins/*chemistry/*metabolism
MH  - *Guanine Nucleotide Exchange Factors
MH  - Humans
MH  - Kinetics
MH  - Nerve Tissue Proteins
MH  - Peptide Elongation Factors/*metabolism/*physiology
MH  - Polymerase Chain Reaction
MH  - Receptors, Transferrin/metabolism
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Sequence Alignment
MH  - Sequence Deletion
MH  - Sequence Homology, Amino Acid
MH  - Transfection
MH  - src Homology Domains
PMC - PMC1171237
EDAT- 1999/03/17 00:00
MHDA- 1999/03/17 00:01
CRDT- 1999/03/17 00:00
PHST- 1999/03/17 00:00 [pubmed]
PHST- 1999/03/17 00:01 [medline]
PHST- 1999/03/17 00:00 [entrez]
AID - 10.1093/emboj/18.6.1480 [doi]
PST - ppublish
SO  - EMBO J. 1999 Mar 15;18(6):1480-91. doi: 10.1093/emboj/18.6.1480.