PMID- 10075738
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 12
DP  - 1999 Mar 19
TI  - Cloning and characterization of androgen receptor coactivator, ARA55, in human
      prostate.
PG  - 8316-21
AB  - Androgen receptor (AR) is a hormone-activated transcriptional factor that can
      bind to androgen response elements and that regulates the transcription of target
      genes via a mechanism that presumably involves cofactors. We report here the
      cloning of a novel AR coactivator ARA55 using a yeast two-hybrid system. ARA55
      consists of 444 amino acids with the predicted molecular mass of 55 kDa and its
      sequence shows very high homology to mouse hic5, a TGF-beta1-inducible gene.
      Yeast and mammalian two-hybrid systems and co-immunoprecipitation assays all
      prove ARA55 can bind to AR in a ligand-dependent manner. Transient transfection
      assay in prostate cancer DU145 cells further demonstrates that ARA55 can enhance 
      AR transcriptional activity in the presence of 1 nM dihydrotestosterone or its
      antagonists such as 100 nM 17beta-estradiol or 1 microM hydroxyflutamide. Our
      data also suggest the C-terminal half of ARA55, which includes three LIM motifs, 
      is sufficient to interact with AR. Northern blot and polymerase chain reaction
      quantitation showed ARA55 can be expressed differently in normal prostate and
      prostate tumor cells. Together, our data suggests that ARA55 may play very
      important roles in the progression of prostate cancer by the modulation of AR
      transactivation.
FAU - Fujimoto, N
AU  - Fujimoto N
AD  - George Whipple Lab for Cancer Research, Departments of Pathology, Urology, and
      Radiation Oncology, University of Rochester Medical Center, Rochester, New York
      14642, USA.
FAU - Yeh, S
AU  - Yeh S
FAU - Kang, H Y
AU  - Kang HY
FAU - Inui, S
AU  - Inui S
FAU - Chang, H C
AU  - Chang HC
FAU - Mizokami, A
AU  - Mizokami A
FAU - Chang, C
AU  - Chang C
LA  - eng
SI  - GENBANK/AF116343
GR  - CA55639/CA/NCI NIH HHS/United States
GR  - CA68568/CA/NCI NIH HHS/United States
GR  - DK51346/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (LIM Domain Proteins)
RN  - 0 (Receptors, Androgen)
RN  - 0 (TGFB1I1 protein, human)
RN  - 0 (Trans-Activators)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - LIM Domain Proteins
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Prostate/*chemistry
MH  - Prostatic Neoplasms/chemistry
MH  - Receptors, Androgen/*metabolism
MH  - Trans-Activators/chemistry/*genetics/isolation & purification/metabolism
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1999/03/13 00:00
MHDA- 1999/03/13 00:01
CRDT- 1999/03/13 00:00
PHST- 1999/03/13 00:00 [pubmed]
PHST- 1999/03/13 00:01 [medline]
PHST- 1999/03/13 00:00 [entrez]
AID - 10.1074/jbc.274.12.8316 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Mar 19;274(12):8316-21. doi: 10.1074/jbc.274.12.8316.