PMID- 10075736
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 12
DP  - 1999 Mar 19
TI  - A novel MDMX transcript expressed in a variety of transformed cell lines encodes 
      a truncated protein with potent p53 repressive activity.
PG  - 8299-308
AB  - The MDMX gene product is related to the MDM2 oncoprotein, both of which interact 
      with the p53 tumor suppressor. We have identified a novel transcript of the MDMX 
      gene that is expressed in a variety of cell lines, and in particular, in growing 
      and transformed cells. This transcript is identical to the published sequence yet
      it has a short internal deletion of 68 base pairs. This deletion produces a shift
      in the reading frame after codon 114, resulting in the inclusion of a stop codon 
      at amino acid residue 127 (full-length MDMX is 489 residues). This truncated MDMX
      protein is termed MDMX-S ("short form"), represents only the p53-binding domain, 
      and appears to bind p53 better than full-length MDMX. The MDMX-S protein can be
      detected in cell extracts and when overexpressed is much more effective than MDMX
      at inhibiting p53-mediated transcriptional activation and induction of apoptosis.
      Since MDMX-S lacks the central and carboxyl-terminal regions contained within
      full-length MDMX, it is likely to play a key role in the regulation of cell
      proliferation and apoptosis in a way distinct from MDMX.
FAU - Rallapalli, R
AU  - Rallapalli R
AD  - Department of Biochemistry and Molecular Pharmacology, Jefferson Cancer
      Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
FAU - Strachan, G
AU  - Strachan G
FAU - Cho, B
AU  - Cho B
FAU - Mercer, W E
AU  - Mercer WE
FAU - Hall, D J
AU  - Hall DJ
LA  - eng
GR  - CA67032/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Nuclear Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 2.3.2.27 (Mdm2 protein, mouse)
RN  - EC 2.3.2.27 (Proto-Oncogene Proteins c-mdm2)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Fluorescent Antibody Technique, Indirect
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Nuclear Proteins
MH  - Open Reading Frames
MH  - Peptide Fragments/genetics/*metabolism
MH  - Proto-Oncogene Proteins/genetics/*metabolism
MH  - Proto-Oncogene Proteins c-mdm2
MH  - Repressor Proteins/genetics/*metabolism
MH  - *Transcription, Genetic
MH  - Transfection
MH  - Tumor Suppressor Protein p53/*genetics
EDAT- 1999/03/13 00:00
MHDA- 1999/03/13 00:01
CRDT- 1999/03/13 00:00
PHST- 1999/03/13 00:00 [pubmed]
PHST- 1999/03/13 00:01 [medline]
PHST- 1999/03/13 00:00 [entrez]
AID - 10.1074/jbc.274.12.8299 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Mar 19;274(12):8299-308. doi: 10.1074/jbc.274.12.8299.