PMID- 10075727
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 12
DP  - 1999 Mar 19
TI  - Characterization of a novel rat brain glycosylphosphatidylinositol-anchored
      protein (Kilon), a member of the IgLON cell adhesion molecule family.
PG  - 8224-30
AB  - In the central nervous system, many cell adhesion molecules are known to
      participate in the establishment and remodeling of the neural circuit. Some of
      the cell adhesion molecules are known to be anchored to the membrane by the
      glycosylphosphatidylinositol (GPI) inserted to their C termini, and many
      GPI-anchored proteins are known to be localized in a Triton-insoluble membrane
      fraction of low density or so-called "raft." In this study, we surveyed the
      GPI-anchored proteins in the Triton-insoluble low density fraction from
      2-week-old rat brain by solubilization with phosphatidylinositol-specific
      phospholipase C. By Western blotting and partial peptide sequencing after the
      deglycosylation with peptide N-glycosidase F, the presence of Thy-1,
      F3/contactin, and T-cadherin was shown. In addition, one of the major proteins,
      having an apparent molecular mass of 36 kDa after the peptide N-glycosidase F
      digestion, was found to be a novel protein. The result of cDNA cloning showed
      that the protein is an immunoglobulin superfamily member with three C2 domains
      and has six putative glycosylation sites. Since this protein shows high sequence 
      similarity to IgLON family members including LAMP, OBCAM, neurotrimin, CEPU-1,
      AvGP50, and GP55, we termed this protein Kilon (a kindred of IgLON).
      Kilon-specific monoclonal antibodies were produced, and Western blotting analysis
      showed that expression of Kilon is restricted to brain, and Kilon has an apparent
      molecular mass of 46 kDa in SDS-polyacrylamide gel electrophoresis in its
      expressed form. In brain, the expression of Kilon is already detected in E16
      stage, and its level gradually increases during development. Kilon immunostaining
      was observed in the cerebral cortex and hippocampus, in which the strongly
      stained puncta were observed on dendrites and soma of pyramidal neurons.
FAU - Funatsu, N
AU  - Funatsu N
AD  - Department of Biotechnology, Kyoto Institute of Technology, Kyoto 606-8585,
      Japan.
FAU - Miyata, S
AU  - Miyata S
FAU - Kumanogoh, H
AU  - Kumanogoh H
FAU - Shigeta, M
AU  - Shigeta M
FAU - Hamada, K
AU  - Hamada K
FAU - Endo, Y
AU  - Endo Y
FAU - Sokawa, Y
AU  - Sokawa Y
FAU - Maekawa, S
AU  - Maekawa S
LA  - eng
SI  - GENBANK/AB017139
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Avian Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Cell Adhesion Molecules, Neuronal)
RN  - 0 (DNA, Complementary)
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (Glycosylphosphatidylinositols)
RN  - 0 (Immunoglobulins)
RN  - 0 (Kilon protein, rat)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (NEGR1 protein, human)
RN  - 0 (Neural Cell Adhesion Molecule L1)
RN  - 0 (Neural Cell Adhesion Molecules)
RN  - 0 (Opcml protein, rat)
RN  - 0 (avian glycoprotein 50-kDa)
RN  - 0 (neural CEPU-1 protein, chicken)
RN  - 0 (neurotrimin)
RN  - EC 3.5.- (Amidohydrolases)
RN  - EC 3.5.1.52 (Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase)
SB  - IM
MH  - Amidohydrolases/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Avian Proteins
MH  - Base Sequence
MH  - *Brain Chemistry
MH  - Carrier Proteins/chemistry
MH  - Cell Adhesion Molecules/chemistry
MH  - Cell Adhesion Molecules, Neuronal/*chemistry/genetics/isolation & purification
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry
MH  - GPI-Linked Proteins
MH  - Glycosylphosphatidylinositols/metabolism
MH  - Immunoglobulins/chemistry
MH  - Membrane Glycoproteins/chemistry
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - *Neural Cell Adhesion Molecule L1
MH  - Neural Cell Adhesion Molecules/chemistry
MH  - Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
MH  - Protein Conformation
MH  - Rats
EDAT- 1999/03/13 00:00
MHDA- 1999/03/13 00:01
CRDT- 1999/03/13 00:00
PHST- 1999/03/13 00:00 [pubmed]
PHST- 1999/03/13 00:01 [medline]
PHST- 1999/03/13 00:00 [entrez]
AID - 10.1074/jbc.274.12.8224 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Mar 19;274(12):8224-30. doi: 10.1074/jbc.274.12.8224.