PMID- 10075724
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 12
DP  - 1999 Mar 19
TI  - Sorting of furin at the trans-Golgi network. Interaction of the cytoplasmic tail 
      sorting signals with AP-1 Golgi-specific assembly proteins.
PG  - 8199-207
AB  - The eukaryotic subtilisin-like endoprotease furin is found predominantly in the
      trans-Golgi network (TGN) and cycles between this compartment, the cell surface, 
      and the endosomes. There is experimental evidence for endocytosis from the plasma
      membrane and transport from endosomes to the TGN, but direct exit from the TGN to
      endosomes via clathrin-coated vesicles has only been discussed but not directly
      shown so far. Here we present data showing that expression of furin promotes the 
      first step of clathrin-coat assembly at the TGN, the recruitment of the
      Golgi-specific assembly protein AP-1 on Golgi membranes. Further, we report that 
      furin indeed is present in isolated clathrin-coated vesicles. Packaging into
      clathrin-coated vesicles requires signal components in the furin cytoplasmic
      domain which can be recognized by AP-1 assembly proteins. We found that besides
      depending on the phosphorylation state of a casein kinase II site, interaction of
      the furin tail with AP-1 and its mu1subunit is mediated by a tyrosine motif and
      to less extent by a leucine-isoleucine signal, whereas a monophenylalanine motif 
      is only involved in binding to the intact AP-1 complex. This study implies that
      high affinity interaction of AP-1 or mu1 with the cytoplasmic tail of furin needs
      a complex interplay of signal components rather than one distinct signal.
FAU - Teuchert, M
AU  - Teuchert M
AD  - Institut fur Virologie der Philipps-Universitat Marburg, Robert-Koch-Strasse 17, 
      35037 Marburg, Germany.
FAU - Schafer, W
AU  - Schafer W
FAU - Berghofer, S
AU  - Berghofer S
FAU - Hoflack, B
AU  - Hoflack B
FAU - Klenk, H D
AU  - Klenk HD
FAU - Garten, W
AU  - Garten W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Clathrin)
RN  - 0 (Transcription Factor AP-1)
RN  - EC 3.4.21.- (Subtilisins)
RN  - EC 3.4.21.75 (Furin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cattle
MH  - Clathrin/metabolism
MH  - Coated Vesicles/metabolism
MH  - Cytoplasm/metabolism
MH  - Escherichia coli
MH  - Furin
MH  - Golgi Apparatus/*metabolism
MH  - Intracellular Membranes/metabolism
MH  - Molecular Sequence Data
MH  - Rabbits
MH  - Structure-Activity Relationship
MH  - Subtilisins/*metabolism
MH  - Transcription Factor AP-1/*metabolism
EDAT- 1999/03/13 00:00
MHDA- 1999/03/13 00:01
CRDT- 1999/03/13 00:00
PHST- 1999/03/13 00:00 [pubmed]
PHST- 1999/03/13 00:01 [medline]
PHST- 1999/03/13 00:00 [entrez]
AID - 10.1074/jbc.274.12.8199 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Mar 19;274(12):8199-207. doi: 10.1074/jbc.274.12.8199.