PMID- 10075717
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 12
DP  - 1999 Mar 19
TI  - Sonic Hedgehog-induced activation of the Gli1 promoter is mediated by GLI3.
PG  - 8143-52
AB  - Drosophila transcription factor cubitus interruptus (Ci) and its co-activator CRE
      (cAMP response element)-binding protein (CBP) activate a group of target genes on
      the anterior-posterior border in response to hedgehog protein (Hh) signaling. In 
      the anterior region, in contrast, the carboxyl-truncated form of Ci generated by 
      protein processing represses Hh expression. In vertebrates, three Ci-related
      transcription factors (glioblastoma gene products (GLIs) 1, 2, and 3) were
      identified, but their functional difference in Hh signal transduction is unknown.
      Here, we report distinct roles for GLI1 and GLI3 in Sonic hedgehog (Shh)
      signaling. GLI3 containing both repression and activation domains acts both as an
      activator and a repressor, as does Ci, whereas GLI1 contains only the activation 
      domain. Consistent with this, GLI3, but not GLI1, is processed to generate the
      repressor form. Transcriptional co-activator CBP binds to GLI3, but not to GLI1. 
      The trans-activating capacity of GLI3 is positively and negatively regulated by
      Shh and cAMP-dependent protein kinase, respectively, through a specific region of
      GLI3, which contains the CBP-binding domain and the phosphorylation sites of
      cAMP-dependent protein kinase. GLI3 directly binds to the Gli1 promoter and
      induces Gli1 transcription in response to Shh. Thus, GLI3 may act as a mediator
      of Shh signaling in the activation of the target gene Gli1.
FAU - Dai, P
AU  - Dai P
AD  - Laboratory of Molecular Genetics, Tsukuba Life Science Center, RIKEN, 3-1-1
      Koyadai, Tsukuba, Ibaraki 305-0074, Japan.
FAU - Akimaru, H
AU  - Akimaru H
FAU - Tanaka, Y
AU  - Tanaka Y
FAU - Maekawa, T
AU  - Maekawa T
FAU - Nakafuku, M
AU  - Nakafuku M
FAU - Ishii, S
AU  - Ishii S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (GLI3 protein, Xenopus)
RN  - 0 (GLI3 protein, human)
RN  - 0 (Gli3 protein, mouse)
RN  - 0 (Hedgehog Proteins)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Oncogene Proteins)
RN  - 0 (Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (SHH protein, human)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (Xenopus Proteins)
RN  - 0 (Zinc Finger Protein GLI1)
RN  - 0 (Zinc Finger Protein Gli3)
RN  - 0 (ci protein, Drosophila)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Consensus Sequence
MH  - DNA-Binding Proteins/*metabolism
MH  - Drosophila Proteins
MH  - Embryonic Induction
MH  - Hedgehog Proteins
MH  - Humans
MH  - Kruppel-Like Transcription Factors
MH  - Mice
MH  - *Nerve Tissue Proteins
MH  - Neurons/metabolism
MH  - Oncogene Proteins/biosynthesis/*genetics
MH  - *Promoter Regions, Genetic
MH  - Proteins/*metabolism
MH  - *Repressor Proteins
MH  - Signal Transduction
MH  - Stem Cells/metabolism
MH  - *Trans-Activators
MH  - Transcription Factors/biosynthesis/*genetics/*metabolism
MH  - Transfection
MH  - *Xenopus Proteins
MH  - Zinc Finger Protein GLI1
MH  - Zinc Finger Protein Gli3
MH  - *Zinc Fingers
EDAT- 1999/03/13 00:00
MHDA- 1999/03/13 00:01
CRDT- 1999/03/13 00:00
PHST- 1999/03/13 00:00 [pubmed]
PHST- 1999/03/13 00:01 [medline]
PHST- 1999/03/13 00:00 [entrez]
AID - 10.1074/jbc.274.12.8143 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Mar 19;274(12):8143-52. doi: 10.1074/jbc.274.12.8143.