PMID- 10075714
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 12
DP  - 1999 Mar 19
TI  - RIN ZF, a novel zinc finger gene, encodes proteins that bind to the CACC element 
      of the gastrin promoter.
PG  - 8123-8
AB  - Expression of gastrin, a gut hormone and growth factor, has tissue-specific
      transcriptional regulation and can be induced in some tumors. Previous studies
      have shown that a CACC cis-regulatory element is important for transcriptional
      activation in pancreatic insulinoma cells. To identify CACC-binding proteins, a
      lambda phage cDNA library derived from a rat insulinoma cell line, RIN 38A, was
      screened by a Southwestern method. A novel member of the Cys2-His2 zinc finger
      gene family was cloned and designated RIN ZF, having a cDNA sequence of 3.8
      kilobases. One full-length and a shorter splice variant were sequenced and had
      predicted protein masses of 91.6 and 88.7 kDa. Expression of both splice forms
      were ubiquitous in fetal and adult rat tissues. Recombinant RIN ZF protein
      exhibited sequence-specific binding to the gastrin CACC element in a gel mobility
      shift assay. In transient transfections, both splice variants appeared to have
      only weak activating effects on gastrin-luciferase reporter gene transcription.
      Furthermore, RIN ZF coexpression with Sp1 appeared to block the strongly
      activating effects of Sp1 mediated through the CACC element. These findings
      suggest that a novel set of zinc finger proteins may help regulate gastrin gene
      expression by interfering with Sp1 transactivation.
FAU - Tillotson, L G
AU  - Tillotson LG
AD  - Division of Digestive Diseases and Nutrition, Department of Medicine, University 
      of North Carolina, Chapel Hill, North Carolina 27599, USA. lgtgi@med.unc.edu
LA  - eng
SI  - GENBANK/AF091457
GR  - R29 DK49860/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Gastrins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Rinzf protein, rat)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/*genetics
MH  - Gastrins/*genetics
MH  - Genes, Reporter
MH  - Insulinoma/genetics
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Polymerase Chain Reaction
MH  - *Promoter Regions, Genetic
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Tumor Cells, Cultured
MH  - Zinc Fingers/*genetics
EDAT- 1999/03/13 00:00
MHDA- 1999/03/13 00:01
CRDT- 1999/03/13 00:00
PHST- 1999/03/13 00:00 [pubmed]
PHST- 1999/03/13 00:01 [medline]
PHST- 1999/03/13 00:00 [entrez]
AID - 10.1074/jbc.274.12.8123 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Mar 19;274(12):8123-8. doi: 10.1074/jbc.274.12.8123.