PMID- 10075713
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 12
DP  - 1999 Mar 19
TI  - Primary structure, tissue distribution, and expression of mouse
      phosphoinositide-dependent protein kinase-1, a protein kinase that phosphorylates
      and activates protein kinase Czeta.
PG  - 8117-22
AB  - Phosphoinositide-dependent protein kinase-1 (PDK1) is a recently identified
      serine/threonine kinase that phosphorylates and activates Akt and p70(S6K), two
      downstream kinases of phosphatidylinositol 3-kinase. To further study the
      potential role of PDK1, we have screened a mouse liver cDNA library and
      identified a cDNA encoding the enzyme. The predicted mouse PDK1 (mPDK1) protein
      contained 559 amino acids and a COOH-terminal pleckstrin homology domain. A
      7-kilobase mPDK1 mRNA was broadly expressed in mouse tissues and in embryonic
      cells. In the testis, a high level expression of a tissue-specific 2-kilobase
      transcript was also detected. Anti-mPDK1 antibody recognized multiple proteins in
      mouse tissues with molecular masses ranging from 60 to 180 kDa. mPDK1
      phosphorylated the conserved threonine residue (Thr402) in the activation loop of
      protein kinase C-zeta and activated the enzyme in vitro and in cells. Our
      findings suggest that there may be different isoforms of mPDK1 and that the
      protein is an upstream kinase that activates divergent pathways downstream of
      phosphatidylinositol 3-kinase.
FAU - Dong, L Q
AU  - Dong LQ
AD  - Department of Pharmacology, The University of Health Science Center, San Antonio,
      Texas 78284, USA.
FAU - Zhang, R B
AU  - Zhang RB
FAU - Langlais, P
AU  - Langlais P
FAU - He, H
AU  - He H
FAU - Clark, M
AU  - Clark M
FAU - Zhu, L
AU  - Zhu L
FAU - Liu, F
AU  - Liu F
LA  - eng
SI  - GENBANK/AF086625
GR  - DK52933/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.1 (3-Phosphoinositide-Dependent Protein Kinases)
RN  - EC 2.7.11.1 (Pdpk1 protein, mouse)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (protein kinase C zeta)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - 3-Phosphoinositide-Dependent Protein Kinases
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Western
MH  - Cloning, Molecular
MH  - Databases, Factual
MH  - Enzyme Activation
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Protein Kinase C/*metabolism
MH  - Protein-Serine-Threonine Kinases/biosynthesis/chemistry/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Signal Transduction
EDAT- 1999/03/13 00:00
MHDA- 1999/03/13 00:01
CRDT- 1999/03/13 00:00
PHST- 1999/03/13 00:00 [pubmed]
PHST- 1999/03/13 00:01 [medline]
PHST- 1999/03/13 00:00 [entrez]
AID - 10.1074/jbc.274.12.8117 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Mar 19;274(12):8117-22. doi: 10.1074/jbc.274.12.8117.