PMID- 10075708
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 12
DP  - 1999 Mar 19
TI  - Genes for the human mitochondrial trifunctional protein alpha- and beta-subunits 
      are divergently transcribed from a common promoter region.
PG  - 8077-84
AB  - Human HADHA and HADHB genes encode the subunits of an enzyme complex, the
      trifunctional protein, involved in mitochondrial beta-oxidation of fatty acids.
      Both genes are located in the same region of chromosome 2p23. We isolated genomic
      clones, including 5' flanking regions, for HADHA and HADHB. Sequencing revealed
      that both of these genes are linked in a head-to-head arrangement on opposite
      strands and have in common a 350-bp 5' flanking region. The 5' flanking region
      has bidirectional promoter activity within this region; two cis elements proved
      critical for the activity. Transcription factor Sp1 functions as an activator for
      the bidirectional promoter by binding to both elements. Therefore, expression of 
      trifunctional protein subunits are probably coordinately regulated by a common
      promoter and by Sp1.
FAU - Orii, K E
AU  - Orii KE
AD  - Department of Pediatrics, Gifu University School of Medicine, Gifu 500-8076,
      Japan. kenjior-gif@umin.u-tokyo.ac.jp
FAU - Orii, K O
AU  - Orii KO
FAU - Souri, M
AU  - Souri M
FAU - Orii, T
AU  - Orii T
FAU - Kondo, N
AU  - Kondo N
FAU - Hashimoto, T
AU  - Hashimoto T
FAU - Aoyama, T
AU  - Aoyama T
LA  - eng
SI  - GENBANK/AB020811
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Multienzyme Complexes)
RN  - 0 (Sp1 Transcription Factor)
RN  - 9007-49-2 (DNA)
RN  - EC 1.1.1.211 (HADHA protein, human)
RN  - EC 1.1.1.211 (Mitochondrial Trifunctional Protein, alpha Subunit)
RN  - EC 2.3.1.16 (HADHB protein, human)
RN  - EC 2.3.1.16 (Mitochondrial Trifunctional Protein)
RN  - EC 2.3.1.16 (Mitochondrial Trifunctional Protein, beta Subunit)
SB  - IM
MH  - Base Sequence
MH  - Cell Line
MH  - Chromosome Mapping
MH  - DNA/chemistry
MH  - DNA Footprinting
MH  - Exons
MH  - Genetic Variation
MH  - HeLa Cells
MH  - Humans
MH  - Mitochondrial Trifunctional Protein
MH  - Mitochondrial Trifunctional Protein, alpha Subunit
MH  - Mitochondrial Trifunctional Protein, beta Subunit
MH  - Molecular Sequence Data
MH  - Multienzyme Complexes/*genetics
MH  - *Promoter Regions, Genetic
MH  - Sp1 Transcription Factor/metabolism
MH  - *Transcription, Genetic
EDAT- 1999/03/13 00:00
MHDA- 1999/03/13 00:01
CRDT- 1999/03/13 00:00
PHST- 1999/03/13 00:00 [pubmed]
PHST- 1999/03/13 00:01 [medline]
PHST- 1999/03/13 00:00 [entrez]
AID - 10.1074/jbc.274.12.8077 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Mar 19;274(12):8077-84. doi: 10.1074/jbc.274.12.8077.