PMID- 10075690
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 12
DP  - 1999 Mar 19
TI  - Inducible degradation of IkappaBalpha by the proteasome requires interaction with
      the F-box protein h-betaTrCP.
PG  - 7941-5
AB  - Activation of NF-kappaB transcription factors requires phosphorylation and
      ubiquitin-proteasome-dependent degradation of IkappaB proteins. We provide
      evidence that a human F-box protein, h-betaTrCP, a component of Skp1-Cullin-F-box
      protein (SCF) complexes, a new class of E3 ubiquitin ligases, is essential for
      inducible degradation of IkappaBalpha. betaTrCP associates with Ser32-Ser36
      phosphorylated, but not with unmodified IkappaBalpha or Ser32-Ser36
      phosphorylation-deficient mutants. Expression of a F-box-deleted betaTrCP
      inhibits IkappaBalpha degradation, promotes accumulation of phosphorylated
      Ser32-Ser36 IkappaBalpha, and prevents NF-kappaB-dependent transcription. Our
      findings indicate that betaTrCP is the adaptor protein required for IkappaBalpha 
      recognition by the SCFbetaTrCP E3 complex that ubiquitinates IkappaBalpha and
      makes it a substrate for the proteasome.
FAU - Kroll, M
AU  - Kroll M
AD  - Unite d'Immunologie Virale, Unite des arbovirus et virus des fievres
      hemorragiques, Institut Pasteur, 25 et 28, rue du Dr. Roux, 75724 Paris Cedex 15,
      France.
FAU - Margottin, F
AU  - Margottin F
FAU - Kohl, A
AU  - Kohl A
FAU - Renard, P
AU  - Renard P
FAU - Durand, H
AU  - Durand H
FAU - Concordet, J P
AU  - Concordet JP
FAU - Bachelerie, F
AU  - Bachelerie F
FAU - Arenzana-Seisdedos, F
AU  - Arenzana-Seisdedos F
FAU - Benarous, R
AU  - Benarous R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (BTRC protein, human)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (NF-kappa B)
RN  - 0 (NFKBIA protein, human)
RN  - 0 (S-Phase Kinase-Associated Proteins)
RN  - 0 (beta-Transducin Repeat-Containing Proteins)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
RN  - 452VLY9402 (Serine)
RN  - EC 2.3.2.27 (SKP Cullin F-Box Protein Ligases)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 6.3.2.- (Peptide Synthases)
SB  - IM
MH  - Cell Cycle Proteins/*metabolism
MH  - Cysteine Endopeptidases/*metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - GTP-Binding Proteins/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - *I-kappa B Proteins
MH  - Models, Chemical
MH  - Multienzyme Complexes/*metabolism
MH  - NF-KappaB Inhibitor alpha
MH  - NF-kappa B/biosynthesis/genetics
MH  - Peptide Synthases/*metabolism
MH  - Phosphorylation
MH  - Proteasome Endopeptidase Complex
MH  - S-Phase Kinase-Associated Proteins
MH  - SKP Cullin F-Box Protein Ligases
MH  - Serine/metabolism
MH  - Transcription, Genetic
MH  - beta-Transducin Repeat-Containing Proteins
EDAT- 1999/03/13 00:00
MHDA- 1999/03/13 00:01
CRDT- 1999/03/13 00:00
PHST- 1999/03/13 00:00 [pubmed]
PHST- 1999/03/13 00:01 [medline]
PHST- 1999/03/13 00:00 [entrez]
AID - 10.1074/jbc.274.12.7941 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Mar 19;274(12):7941-5. doi: 10.1074/jbc.274.12.7941.