PMID- 10075682
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 12
DP  - 1999 Mar 19
TI  - TWIK-2, a new weak inward rectifying member of the tandem pore domain potassium
      channel family.
PG  - 7887-92
AB  - Potassium channels are found in all mammalian cell types, and they perform many
      distinct functions in both excitable and non-excitable cells. These functions are
      subserved by several different families of potassium channels distinguishable by 
      primary sequence features as well as by physiological characteristics. Of these
      families, the tandem pore domain potassium channels are a new and distinct class,
      primarily distinguished by the presence of two pore-forming domains within a
      single polypeptide chain. We have cloned a new member of this family, TWIK-2,
      from a human brain cDNA library. Primary sequence analysis of TWIK-2 shows that
      it is most closely related to TWIK-1, especially in the pore-forming domains.
      Northern blot analysis reveals the expression of TWIK-2 in all human tissues
      assayed except skeletal muscle. Human TWIK-2 expressed heterologously in Xenopus 
      oocytes is a non-inactivating weak inward rectifier with channel properties
      similar to TWIK-1. Pharmacologically, TWIK-2 channels are distinct from TWIK-1
      channels in their response to quinidine, quinine, and barium. TWIK-2 is inhibited
      by intracellular, but not extracellular, acidification. This new clone reveals
      the existence of a subfamily in the tandem pore domain potassium channel family
      with weak inward rectification properties.
FAU - Chavez, R A
AU  - Chavez RA
AD  - Elan Pharmaceuticals, Menlo Park, California 94025, USA.
FAU - Gray, A T
AU  - Gray AT
FAU - Zhao, B B
AU  - Zhao BB
FAU - Kindler, C H
AU  - Kindler CH
FAU - Mazurek, M J
AU  - Mazurek MJ
FAU - Mehta, Y
AU  - Mehta Y
FAU - Forsayeth, J R
AU  - Forsayeth JR
FAU - Yost, C S
AU  - Yost CS
LA  - eng
SI  - GENBANK/AF117708
GR  - GM51372/GM/NIGMS NIH HHS/United States
GR  - GM57740/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (KCNK6 protein, human)
RN  - 0 (Potassium Channels)
RN  - 0 (Potassium Channels, Tandem Pore Domain)
RN  - 24GP945V5T (Barium)
SB  - IM
EIN - J Biol Chem 1999 Aug 20;274(34):24440
MH  - Amino Acid Sequence
MH  - Animals
MH  - Barium/pharmacology
MH  - Base Sequence
MH  - Blotting, Northern
MH  - *Brain Chemistry
MH  - Cloning, Molecular
MH  - Glycosylation
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Molecular Sequence Data
MH  - Potassium Channels/*chemistry/*genetics/metabolism
MH  - Potassium Channels, Tandem Pore Domain
MH  - Sequence Alignment
MH  - Xenopus
EDAT- 1999/03/13 00:00
MHDA- 1999/03/13 00:01
CRDT- 1999/03/13 00:00
PHST- 1999/03/13 00:00 [pubmed]
PHST- 1999/03/13 00:01 [medline]
PHST- 1999/03/13 00:00 [entrez]
AID - 10.1074/jbc.274.12.7887 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Mar 19;274(12):7887-92. doi: 10.1074/jbc.274.12.7887.