PMID- 10075675
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 12
DP  - 1999 Mar 19
TI  - Sec24 proteins and sorting at the endoplasmic reticulum.
PG  - 7833-40
AB  - COPII proteins are necessary to generate secretory vesicles at the endoplasmic
      reticulum. In yeast, the Sec24p protein is the only COPII component in which two 
      close orthologues have been identified. By using gene knock-out in yeast, we
      found that the absence of one of these Sec24 orthologues resulted in a selective 
      secretion defect for a subset of proteins released into the medium. Data base
      searches revealed the existence of an entire family of Sec24-related proteins in 
      humans, worms, flies, and plants. We identified and cloned two new human cDNAs
      encoding proteins homologous to yeast Sec24p, in addition to two human cDNAs
      already present within the data bases. The entire Sec24 family identified to date
      is characterized by clusters of highly conserved residues within the 2/3
      carboxyl-terminal domain of all the proteins and a divergent amino terminus
      domain. Human (h) Sec24 orthologues co-immunoprecipitate with hSec23Ap and
      migrate as a complex by size exclusion chromatography. Immunofluorescence
      microscopy confirmed that these proteins co-localize with hSec23p and hSec13p.
      Together, our data suggest that in addition to its role in the shaping up of the 
      vesicle, the Sec23-24p complex may be implicated in cargo selection and
      concentration.
FAU - Pagano, A
AU  - Pagano A
AD  - Department of Morphology, University Medical Center, Geneva University, Geneva
      CH-1211, Switzerland.
FAU - Letourneur, F
AU  - Letourneur F
FAU - Garcia-Estefania, D
AU  - Garcia-Estefania D
FAU - Carpentier, J L
AU  - Carpentier JL
FAU - Orci, L
AU  - Orci L
FAU - Paccaud, J P
AU  - Paccaud JP
LA  - eng
SI  - GENBANK/AJ131244
SI  - GENBANK/AJ131245
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (LMAN1 protein, human)
RN  - 0 (Mannose-Binding Lectins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proteins)
RN  - 0 (SEC23A protein, human)
RN  - 0 (SEC24 protein, S cerevisiae)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Vesicular Transport Proteins)
RN  - 0 (sec-23 protein, C elegans)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Arabidopsis
MH  - Caenorhabditis elegans
MH  - Caenorhabditis elegans Proteins
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - DNA/metabolism
MH  - Drosophila melanogaster
MH  - Endoplasmic Reticulum/*physiology
MH  - Humans
MH  - *Mannose-Binding Lectins
MH  - Membrane Proteins/genetics/metabolism/physiology
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Proteins/genetics/physiology
MH  - Saccharomyces cerevisiae
MH  - *Saccharomyces cerevisiae Proteins
MH  - Sequence Alignment
MH  - Vesicular Transport Proteins
EDAT- 1999/03/13 00:00
MHDA- 1999/03/13 00:01
CRDT- 1999/03/13 00:00
PHST- 1999/03/13 00:00 [pubmed]
PHST- 1999/03/13 00:01 [medline]
PHST- 1999/03/13 00:00 [entrez]
AID - 10.1074/jbc.274.12.7833 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Mar 19;274(12):7833-40. doi: 10.1074/jbc.274.12.7833.