PMID- 10075656
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 12
DP  - 1999 Mar 19
TI  - p300 interacts with the N- and C-terminal part of PPARgamma2 in a
      ligand-independent and -dependent manner, respectively.
PG  - 7681-8
AB  - The nuclear peroxisome proliferator-activated receptor gamma (PPARgamma)
      activates the transcription of multiple genes involved in intra- and
      extracellular lipid metabolism. Several cofactors are crucial for the stimulation
      or the silencing of nuclear receptor transcriptional activities. The two
      homologous cofactors p300 and CREB-binding protein (CBP) have been shown to
      co-activate the ligand-dependent transcriptional activities of several nuclear
      receptors as well as the ligand-independent transcriptional activity of the
      androgen receptor. We show here that the interaction between p300/CBP and
      PPARgamma is complex and involves multiple domains in each protein. p300/CBP not 
      only bind in a ligand-dependent manner to the DEF region of PPARgamma but also
      bind directly in a ligand-independent manner to a region in the AB domain
      localized between residue 31 to 99. In transfection experiments, p300/CBP could
      thereby enhance the transcriptional activities of both the activating function
      (AF)-1 and AF-2 domains. p300/CBP displays itself at least two docking sites for 
      PPARgamma located in its N terminus (between residues 1 and 113 for CBP) and in
      the middle of the protein (between residues 1099 and 1460).
FAU - Gelman, L
AU  - Gelman L
AD  - Unite 325 INSERM, Departement d'Atherosclerose, Institut Pasteur de Lille, 1, rue
      du Prof. Calmette, 59019 Lille Cedex, France.
FAU - Zhou, G
AU  - Zhou G
FAU - Fajas, L
AU  - Fajas L
FAU - Raspe, E
AU  - Raspe E
FAU - Fruchart, J C
AU  - Fruchart JC
FAU - Auwerx, J
AU  - Auwerx J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Ligands)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Thiazoles)
RN  - 0 (Thiazolidinediones)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 05V02F2KDG (Rosiglitazone)
SB  - IM
MH  - Cyclic AMP Response Element-Binding Protein/metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Ligands
MH  - Nuclear Proteins/*metabolism
MH  - Receptors, Cytoplasmic and Nuclear/*metabolism
MH  - Rosiglitazone
MH  - Thiazoles/pharmacology
MH  - *Thiazolidinediones
MH  - Trans-Activators/*metabolism
MH  - Transcription Factors/*metabolism
MH  - Zinc Fingers
EDAT- 1999/03/13 00:00
MHDA- 1999/03/13 00:01
CRDT- 1999/03/13 00:00
PHST- 1999/03/13 00:00 [pubmed]
PHST- 1999/03/13 00:01 [medline]
PHST- 1999/03/13 00:00 [entrez]
AID - 10.1074/jbc.274.12.7681 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Mar 19;274(12):7681-8. doi: 10.1074/jbc.274.12.7681.