PMID- 10074429
OWN - NLM
STAT- MEDLINE
DCOM- 19990422
LR  - 20220214
IS  - 0960-9822 (Print)
IS  - 0960-9822 (Linking)
VI  - 9
IP  - 4
DP  - 1999 Feb 25
TI  - Self-regulated polymerization of the actin-related protein Arp1.
PG  - 223-6
AB  - The actin-related protein Arp1 (or centractin, actin RPV) is the major subunit of
      dynactin, a key component of the cytoplasmic dynein motor machinery [1] [2] [3]. 
      Of the ubiquitously expressed members of the Arp superfamily, Arp1 is most
      similar to conventional actin [4] [5] [6] and, on the basis of conserved sequence
      features, is predicted to bind ATP and possibly polymerize. In vivo, all
      cytosolic Arp1 sediments at 20S [7] suggesting that it assembles into oligomers, 
      most likely dynactin - a multiprotein complex known to contain eight or nine Arp1
      monomers in a 37 nm filament [8]. The uniform length of Arp1 polymers suggests a 
      novel assembly mechanism that may be governed by a 'ruler' activity. In dynactin,
      the Arp1 filament is bounded by actin-capping protein at one end and a
      heterotetrameric protein complex containing the p62 subunit (D.M. Eckley, S.R.
      Gill, J.B.B., J.E. Heuser, T.A.S., unpublished observations) at the other [8]. In
      the present study, we analyzed the behavior of highly purified, native Arp1. Arp1
      was found to polymerize rapidly into short filaments that were similar, but not
      identical, in length to those in dynactin. With time, these filaments appeared to
      anneal to form longer assemblies but never attained the length of conventional
      actin filaments.
FAU - Bingham, J B
AU  - Bingham JB
AD  - Department of Biology The Johns Hopkins University Baltimore Maryland 21218 USA.
FAU - Schroer, T A
AU  - Schroer TA
LA  - eng
GR  - R01 GM044589/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Curr Biol
JT  - Current biology : CB
JID - 9107782
RN  - 0 (Actins)
RN  - 0 (Dinucleoside Phosphates)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Multiprotein Complexes)
RN  - 148996-73-0 (centractin)
RN  - 415SHH325A (Adenosine Monophosphate)
RN  - 50304-44-4 (P(1),P(5)-di(adenosine-5'-)pentaphosphate)
RN  - 61D2G4IYVH (Adenosine Diphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Actins/*chemistry/metabolism/*ultrastructure
MH  - Adenosine Diphosphate/pharmacology
MH  - Adenosine Monophosphate/pharmacology
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Brain Chemistry
MH  - Cattle
MH  - Dinucleoside Phosphates/pharmacology
MH  - Homeostasis
MH  - Kinetics
MH  - Macromolecular Substances
MH  - Microscopy, Electron
MH  - Multiprotein Complexes
MH  - Protein Structure, Secondary
EDAT- 1999/03/13 00:00
MHDA- 1999/03/13 00:01
CRDT- 1999/03/13 00:00
PHST- 1999/03/13 00:00 [pubmed]
PHST- 1999/03/13 00:01 [medline]
PHST- 1999/03/13 00:00 [entrez]
AID - S0960-9822(99)80095-5 [pii]
AID - 10.1016/s0960-9822(99)80095-5 [doi]
PST - ppublish
SO  - Curr Biol. 1999 Feb 25;9(4):223-6. doi: 10.1016/s0960-9822(99)80095-5.