PMID- 10073577
OWN - NLM
STAT- MEDLINE
DCOM- 19990316
LR  - 20191217
IS  - 1044-5498 (Print)
IS  - 1044-5498 (Linking)
VI  - 18
IP  - 2
DP  - 1999 Feb
TI  - Identification of mouse CPX-1, a novel member of the metallocarboxypeptidase gene
      family with highest similarity to CPX-2.
PG  - 175-85
AB  - The recent finding that Cpe(fat)/Cpe(fat) mice, which lack carboxypeptidase E
      (CPE) activity because of a point mutation, are still capable of a reduced amount
      of neuroendocrine peptide processing suggested that additional carboxypeptidases 
      (CPs) participate in this processing reaction. Searches for novel members of the 
      CPE gene family led to the discovery of CPD, CPZ, AEBP1, and CPX-2. In the
      present report, we describe mouse CPX-1, another novel member of this gene
      family. Like AEBP1 and CPX-2, CPX-1 contains an N-terminal region of 160 amino
      acids with sequence similarity to the discoidin domain of a variety of proteins. 
      The 410-residue CP-like domain of CPX-1 has 54% to 62% amino acid sequence
      identity with AEBP1 and CPX-2 and 33% to 49% amino acid identity with other
      members of the CPE subfamily. However, several active-site residues that are
      important for catalytic activity of other CPs are not conserved in CPX-1.
      Furthermore, CPX-1 expressed in either the baculovirus system or the mouse AtT-20
      cell line does not cleave standard CP substrates. Northern blot analysis showed
      the highest levels of CPX-1 mRNA in testis and spleen and lower levels in
      salivary gland, brain, heart, lung, and kidney. In situ hybridization of CPX-1
      mRNA in embryonic and fetal mouse tissue showed expression throughout the head
      and thorax, with abundance in primordial cartilage and skeletal structures. In
      the head, high levels of CPX-1 mRNA were associated with the nasal mesenchyme,
      primordial cartilage structures in the ear, and the meninges. In the thorax,
      CPX-1 mRNA was expressed in multiple developing skeletal structures, including
      chondrocytes and perichondrial cells of the rib, vertebral, and long-bone
      primordia. Taken together, these findings suggest that it is unlikely that CPX-1 
      functions in the processing of neuroendocrine peptides. Instead, CPX-1 may have a
      role in development, possibly mediating cell interactions via its discoidin
      domain.
FAU - Lei, Y
AU  - Lei Y
AD  - Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx,
      New York 10461, USA.
FAU - Xin, X
AU  - Xin X
FAU - Morgan, D
AU  - Morgan D
FAU - Pintar, J E
AU  - Pintar JE
FAU - Fricker, L D
AU  - Fricker LD
LA  - eng
SI  - GENBANK/AF077738
GR  - DA-00194/DA/NIDA NIH HHS/United States
GR  - DA-04494/DA/NIDA NIH HHS/United States
GR  - DK-51271/DK/NIDDK NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.- (Carboxypeptidases)
RN  - EC 3.4.- (Cpxm2 protein, mouse)
RN  - EC 3.4.- (Metalloexopeptidases)
RN  - EC 3.4.17.- (Cpxm1 protein, mouse)
RN  - EC 3.4.17.1 (Carboxypeptidases A)
RN  - EC 3.4.24.- (Metalloendopeptidases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Carboxypeptidases/*genetics
MH  - *Carboxypeptidases A
MH  - Carrier Proteins/*genetics
MH  - Cell Line
MH  - DNA, Complementary/chemistry/genetics
MH  - Female
MH  - Gene Expression
MH  - Genes/*genetics
MH  - Humans
MH  - *Metalloendopeptidases
MH  - *Metalloexopeptidases
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Pregnancy
MH  - RNA, Messenger/genetics/metabolism
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
MH  - Tumor Cells, Cultured/cytology/metabolism
EDAT- 1999/03/12 00:00
MHDA- 1999/03/12 00:01
CRDT- 1999/03/12 00:00
PHST- 1999/03/12 00:00 [pubmed]
PHST- 1999/03/12 00:01 [medline]
PHST- 1999/03/12 00:00 [entrez]
AID - 10.1089/104454999315565 [doi]
PST - ppublish
SO  - DNA Cell Biol. 1999 Feb;18(2):175-85. doi: 10.1089/104454999315565.