PMID- 10072773
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 228
IP  - 1-2
DP  - 1999 Mar 4
TI  - Identification of ZFR, an ancient and highly conserved murine
      chromosome-associated zinc finger protein.
PG  - 197-211
AB  - In a screen for RNA binding proteins expressed during murine spermatogenesis, we 
      cloned a novel, ancient zinc finger protein possessing a region common to a small
      class of RNA binding proteins. Zfr (zinc finger RNA binding) encodes a protein of
      1052 amino acids with three widely spaced Cys2His2 zinc fingers. Outside of the
      zinc fingers, ZFR shares a region that is highly conserved between several RNA
      binding proteins containing copies of the double-stranded RNA binding motif. By
      northern blotting, Zfr is expressed at highest levels within the testis, ovary
      and brain. Immunohistochemistry and confocal microscopy were used to show that
      ZFR is highly expressed during meiosis I in males and females and is chromosome
      associated. Zfr is also expressed in Sertoli cells in the testis and granulosa
      cells in the ovary where it is localized to the nucleus. Using fluorescent in
      situ hybridization we mapped Zfr to chromosome 15 region A. ZFR appears to be an 
      ancient protein, as apparent homologs exist in invertebrates (D. melanogaster)
      nematodes (C. elegans) and humans (H. sapiens).
FAU - Meagher, M J
AU  - Meagher MJ
AD  - Department of Genetics, University of Washington, Seattle, WA 98195, USA.
FAU - Schumacher, J M
AU  - Schumacher JM
FAU - Lee, K
AU  - Lee K
FAU - Holdcraft, R W
AU  - Holdcraft RW
FAU - Edelhoff, S
AU  - Edelhoff S
FAU - Disteche, C
AU  - Disteche C
FAU - Braun, R E
AU  - Braun RE
LA  - eng
SI  - GENBANK/AF071059
GR  - GM46883/GM/NIGMS NIH HHS/United States
GR  - HD27215/HD/NICHD NIH HHS/United States
GR  - HD95003/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA, Complementary)
RN  - 0 (Nucleic Acids)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (ZFR protein, human)
RN  - 0 (ZFR protein, mouse)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Western
MH  - Caenorhabditis elegans
MH  - Chromosome Mapping
MH  - Chromosomes/*genetics
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - DNA, Complementary/chemistry/genetics/isolation & purification
MH  - Drosophila melanogaster
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nucleic Acids/metabolism
MH  - Ovary/chemistry
MH  - Protein Binding
MH  - RNA/genetics/metabolism
MH  - RNA-Binding Proteins/*genetics/metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Spermatocytes/metabolism
MH  - Testis/chemistry
MH  - Tissue Distribution
MH  - Transcription, Genetic
MH  - Zinc Fingers/*genetics
EDAT- 1999/03/12 00:00
MHDA- 1999/03/12 00:01
CRDT- 1999/03/12 00:00
PHST- 1999/03/12 00:00 [pubmed]
PHST- 1999/03/12 00:01 [medline]
PHST- 1999/03/12 00:00 [entrez]
AID - S0378-1119(98)00615-5 [pii]
AID - 10.1016/s0378-1119(98)00615-5 [doi]
PST - ppublish
SO  - Gene. 1999 Mar 4;228(1-2):197-211. doi: 10.1016/s0378-1119(98)00615-5.