PMID- 10072771
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 228
IP  - 1-2
DP  - 1999 Mar 4
TI  - Characterization of a murine gene homologous to the bovine CaCC chloride channel.
PG  - 181-8
AB  - The bovine CaCC protein is a putative Ca2+-dependent Cl- channel of airway
      epithelial cells. Therefore, CaCC proteins could contribute to transepithelial
      Cl- transport and accordingly modify the phenotype of cystic fibrosis (CF)
      patients. We have identified a murine EST containing a full-length cDNA coding
      for a 902-amino-acid protein highly homologous to bovine CaCC. The murine gene
      (mCaCC) maps to chromosome 3 at the H2-H3 band and is expressed, as indicated by 
      Northern blot analysis, in mouse skin and kidney but not in brain, heart, lung or
      testis. RT-PCR indicates a low expression in tracheal epithelial cells.
      Heterologous expression of mCaCC in Xenopus oocytes elicits membrane currents
      that are anion-selective and inhibited by DIDS and by niflumic acid, a blocker of
      the endogenous chloride current in oocytes. The identification of genes belonging
      to the CaCC family will help to evaluate their role as ion channels or channel
      regulators and their actual contribution to epithelial chloride transport.
FAU - Romio, L
AU  - Romio L
AD  - Laboratorio di Genetica Molecolare, Istituto Giannina Gaslini, Genoa 16148,
      Italy.
FAU - Musante, L
AU  - Musante L
FAU - Cinti, R
AU  - Cinti R
FAU - Seri, M
AU  - Seri M
FAU - Moran, O
AU  - Moran O
FAU - Zegarra-Moran, O
AU  - Zegarra-Moran O
FAU - Galietta, L J
AU  - Galietta LJ
LA  - eng
SI  - GENBANK/AF052746
GR  - E.0593/Telethon/Italy
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Chloride Channels)
RN  - 0 (Ionophores)
RN  - 0 (RNA, Messenger)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - 4U5MP5IUD8 (Niflumic Acid)
RN  - 56092-81-0 (Ionomycin)
RN  - Q1O6DSW23R (4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid/pharmacology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Northern
MH  - Calcium/*physiology
MH  - Cattle
MH  - Chloride Channels/administration & dosage/*genetics
MH  - Chromosome Mapping
MH  - Expressed Sequence Tags
MH  - Female
MH  - Gene Expression
MH  - Genes/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Ionomycin/pharmacology
MH  - Ionophores/pharmacology
MH  - Male
MH  - Membrane Potentials/drug effects
MH  - Mice
MH  - Microinjections
MH  - Molecular Sequence Data
MH  - Niflumic Acid/pharmacology
MH  - Oocytes/cytology/drug effects/physiology
MH  - RNA, Messenger/genetics/metabolism
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Sodium Chloride/pharmacology
MH  - Tissue Distribution
MH  - Xenopus
EDAT- 1999/03/12 00:00
MHDA- 1999/03/12 00:01
CRDT- 1999/03/12 00:00
PHST- 1999/03/12 00:00 [pubmed]
PHST- 1999/03/12 00:01 [medline]
PHST- 1999/03/12 00:00 [entrez]
AID - S0378-1119(98)00620-9 [pii]
AID - 10.1016/s0378-1119(98)00620-9 [doi]
PST - ppublish
SO  - Gene. 1999 Mar 4;228(1-2):181-8. doi: 10.1016/s0378-1119(98)00620-9.