PMID- 10072761
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 228
IP  - 1-2
DP  - 1999 Mar 4
TI  - Characterisation and mitochondrial localisation of AUH, an AU-specific
      RNA-binding enoyl-CoA hydratase.
PG  - 85-91
AB  - AU-rich elements function as instability elements which direct rapid mRNA
      degradation. AUH protein exhibits an AU-specific RNA-binding property and an
      intrinsic enoyl-CoA hydratase activity and may therefore function to link mRNA
      decay to metabolic processes (. Proc. Natl. Acad. Sci. USA 92, 2051-2055). The
      sequence encoding the murine protein, muAUH, was established by cloning, and the 
      corresponding polypeptide predicted to have a molecular mass of 37kDa. As shown
      for the human protein, muAUH is expressed in a 32kDa form and there is 94%
      homology between the two species. Recombinant muAUH was shown to be an
      RNA-binding enoyl-CoA hydratase. All murine cells studied contained a single AUH 
      transcript of approx. 1.7kb and an investigation of tissue-specific expression
      revealed highest levels in kidney, skeletal muscle, heart, liver and spleen. It
      was further determined, using immunoelectron microscopy, that AUH is located in
      the mitochondria of mouse cells.
FAU - Brennan, L E
AU  - Brennan LE
AD  - Institute for Medical Microbiology, University of Basel, Petersplatz 10, CH-4003,
      Basel, Switzerland.
FAU - Nakagawa, J
AU  - Nakagawa J
FAU - Egger, D
AU  - Egger D
FAU - Bienz, K
AU  - Bienz K
FAU - Moroni, C
AU  - Moroni C
LA  - eng
SI  - GENBANK/AF118386
PT  - Journal Article
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - EC 4.2.1.17 (AUH protein, human)
RN  - EC 4.2.1.17 (Enoyl-CoA Hydratase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - DNA, Complementary/chemistry/genetics/isolation & purification
MH  - Enoyl-CoA Hydratase/analysis/*genetics
MH  - Fluorescent Antibody Technique
MH  - Gene Expression Regulation, Enzymologic
MH  - Humans
MH  - Kidney/enzymology
MH  - Male
MH  - Mast Cells/cytology/enzymology/ultrastructure
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mitochondria/*enzymology/ultrastructure
MH  - Molecular Sequence Data
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA-Binding Proteins/analysis/*genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
EDAT- 1999/03/12 00:00
MHDA- 1999/03/12 00:01
CRDT- 1999/03/12 00:00
PHST- 1999/03/12 00:00 [pubmed]
PHST- 1999/03/12 00:01 [medline]
PHST- 1999/03/12 00:00 [entrez]
AID - S0378-1119(99)00003-7 [pii]
AID - 10.1016/s0378-1119(99)00003-7 [doi]
PST - ppublish
SO  - Gene. 1999 Mar 4;228(1-2):85-91. doi: 10.1016/s0378-1119(99)00003-7.