PMID- 10072760
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 228
IP  - 1-2
DP  - 1999 Mar 4
TI  - Isolation and characterization of IPP, a novel human gene encoding an
      actin-binding, kelch-like protein.
PG  - 73-83
AB  - The kelch family of proteins is defined by a 50 amino-acid repeat that has been
      shown to associate with actin. Here we describe the cloning and initial
      characterization of IPP, a novel human gene that predicts a kelch family protein 
      homologous to the mouse Ipp gene, a previously described kelch family member. A
      3kb IPP cDNA clone was isolated from a human placenta library using a probe
      derived from Ipp. Restriction mapping and Southern blot analysis show that IPP
      comprises eight exons spanning more than 47kb of genomic DNA. Fluorescence in
      situ hybridization maps the gene to chromosome 1p32-1p34. Northern blot analysis 
      reveals transcripts of 1.4, 2.2, 5. 0, and 7.3kb. The 1.4 and 2.2kb messages are 
      found exclusively in testis, while the 5.0 and 7.3kb messages are expressed at
      varying levels in ovary, placenta, small intestine, spleen, testis, and thymus.
      The IPP cDNA clone contains a 1752bp open reading frame that predicts a 584
      amino-acid, 66kDa protein. Sequence analysis indicates an N-terminal POZ
      protein-protein interaction domain and a C-terminal kelch repeat domain
      consisting of six tandemly arranged repeats. Cosedimentation assays performed
      with these domains expressed as glutathione S-transferase fusion proteins
      demonstrate an actin-binding activity mediated specifically by the kelch repeat
      domain of IPP.
FAU - Kim, I F
AU  - Kim IF
AD  - The Johns Hopkins University, Department of Biology, 3400 North Charles Street,
      Baltimore, MD 21218, USA.
FAU - Mohammadi, E
AU  - Mohammadi E
FAU - Huang, R C
AU  - Huang RC
LA  - eng
SI  - GENBANK/AF156857
GR  - 5RO1CA48263/CA/NCI NIH HHS/United States
GR  - 5T32GM07231/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Actins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (IPP protein, human)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Pregnancy Proteins)
RN  - 0 (RNA, Messenger)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Actins/metabolism
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Binding Sites
MH  - Blotting, Northern
MH  - Carrier Proteins/*genetics/metabolism
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 1/genetics
MH  - Cytoskeletal Proteins
MH  - DNA/chemistry/genetics/isolation & purification
MH  - DNA, Complementary/chemistry/genetics/isolation & purification
MH  - Female
MH  - Genes/*genetics
MH  - HeLa Cells
MH  - Humans
MH  - Male
MH  - Microfilament Proteins/*genetics
MH  - Molecular Sequence Data
MH  - Pregnancy Proteins/*genetics
MH  - Protein Binding
MH  - RNA, Messenger/genetics/metabolism
MH  - Repetitive Sequences, Nucleic Acid
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
EDAT- 1999/03/12 00:00
MHDA- 1999/03/12 00:01
CRDT- 1999/03/12 00:00
PHST- 1999/03/12 00:00 [pubmed]
PHST- 1999/03/12 00:01 [medline]
PHST- 1999/03/12 00:00 [entrez]
AID - S0378-1119(99)00006-2 [pii]
AID - 10.1016/s0378-1119(99)00006-2 [doi]
PST - ppublish
SO  - Gene. 1999 Mar 4;228(1-2):73-83. doi: 10.1016/s0378-1119(99)00006-2.