PMID- 10072072
OWN - NLM
STAT- MEDLINE
DCOM- 19990325
LR  - 20071114
IS  - 1074-7613 (Print)
IS  - 1074-7613 (Linking)
VI  - 10
IP  - 2
DP  - 1999 Feb
TI  - Cathepsin S required for normal MHC class II peptide loading and germinal center 
      development.
PG  - 197-206
AB  - Major histocompatibility complex (MHC) class II molecules acquire antigenic
      peptides after degradation of the invariant chain (Ii), an MHC class
      II-associated protein that otherwise blocks peptide binding. Antigen-presenting
      cells of mice that lack the protease cathepsin S fail to process Ii beyond a 10
      kDa fragment, resulting in delayed peptide loading and accumulation of cell
      surface MHC class II/10 kDa Ii complexes. Although cathepsin S-deficient mice
      have normal numbers of B and T cells and normal IgE responses, they show markedly
      impaired antibody class switching to IgG2a and IgG3. These results indicate
      cathepsin S is a major Ii-processing enzyme in splenocytes and dendritic cells.
      Its role in humoral immunity critically depends on how antigens access the immune
      system.
FAU - Shi, G P
AU  - Shi GP
AD  - Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, 
      Boston, Massachusetts 02115, USA.
FAU - Villadangos, J A
AU  - Villadangos JA
FAU - Dranoff, G
AU  - Dranoff G
FAU - Small, C
AU  - Small C
FAU - Gu, L
AU  - Gu L
FAU - Haley, K J
AU  - Haley KJ
FAU - Riese, R
AU  - Riese R
FAU - Ploegh, H L
AU  - Ploegh HL
FAU - Chapman, H A
AU  - Chapman HA
LA  - eng
GR  - AI34893/AI/NIAID NIH HHS/United States
GR  - CA74886/CA/NCI NIH HHS/United States
GR  - HL48716/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Peptides)
RN  - 0 (invariant chain)
RN  - 9007-81-2 (Freund's Adjuvant)
RN  - EC 3.4.- (Cathepsins)
RN  - EC 3.4.22.27 (cathepsin S)
SB  - IM
MH  - Animals
MH  - Antibody Formation
MH  - Antigen Presentation
MH  - Antigens, Differentiation, B-Lymphocyte/metabolism
MH  - Cathepsins/*physiology
MH  - Chimera
MH  - Dendritic Cells/immunology
MH  - Freund's Adjuvant/immunology
MH  - Germinal Center/*physiology
MH  - Histocompatibility Antigens Class II/*metabolism
MH  - Immunization
MH  - Immunoglobulin Class Switching
MH  - Mice
MH  - Mice, Mutant Strains
MH  - Peptides/*metabolism
MH  - Spleen/immunology
EDAT- 1999/03/11 00:00
MHDA- 1999/03/11 00:01
CRDT- 1999/03/11 00:00
PHST- 1999/03/11 00:00 [pubmed]
PHST- 1999/03/11 00:01 [medline]
PHST- 1999/03/11 00:00 [entrez]
AID - S1074-7613(00)80020-5 [pii]
PST - ppublish
SO  - Immunity. 1999 Feb;10(2):197-206.