PMID- 10072071
OWN - NLM
STAT- MEDLINE
DCOM- 19990325
LR  - 20190915
IS  - 1074-7613 (Print)
IS  - 1074-7613 (Linking)
VI  - 10
IP  - 2
DP  - 1999 Feb
TI  - Deficiency of the hematopoietic cell-specific Rho family GTPase Rac2 is
      characterized by abnormalities in neutrophil function and host defense.
PG  - 183-96
AB  - In mammals, the Rho family GTPase Rac2 is restricted in expression to
      hematopoietic cells, where it is coexpressed with Rac1. Rac2-deficient mice were 
      created to define the physiological requirement for two near-identical Rac
      proteins in hematopoietic cells. rac2-/- neutrophils displayed significant
      defects in chemotaxis, in shear-dependent L-selectin-mediated capture on the
      endothelial substrate Glycam-1, and in both F-actin generation and p38 and,
      unexpectedly, p42/p44 MAP kinase activation induced by chemoattractants.
      Superoxide production by rac2-/- bone marrow neutrophils was significantly
      reduced compared to wild type, but it was normal in activated peritoneal exudate 
      neutrophils. These defects were reflected in vivo by baseline neutrophilia,
      reduced inflammatory peritoneal exudate formation, and increased mortality when
      challenged with Aspergillus fumigatus. Rac2 is an essential regulator of multiple
      specialized neutrophil functions.
FAU - Roberts, A W
AU  - Roberts AW
AD  - Howard Hughes Medical Institute, and Herman B Wells Center for Pediatric
      Research, Department of Pediatrics, Indiana University School of Medicine,
      Indianapolis 46202, USA.
FAU - Kim, C
AU  - Kim C
FAU - Zhen, L
AU  - Zhen L
FAU - Lowe, J B
AU  - Lowe JB
FAU - Kapur, R
AU  - Kapur R
FAU - Petryniak, B
AU  - Petryniak B
FAU - Spaetti, A
AU  - Spaetti A
FAU - Pollock, J D
AU  - Pollock JD
FAU - Borneo, J B
AU  - Borneo JB
FAU - Bradford, G B
AU  - Bradford GB
FAU - Atkinson, S J
AU  - Atkinson SJ
FAU - Dinauer, M C
AU  - Dinauer MC
FAU - Williams, D A
AU  - Williams DA
LA  - eng
GR  - 1P01CA71932/CA/NCI NIH HHS/United States
GR  - R01 45635/PHS HHS/United States
GR  - R01 HL52565/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (Actins)
RN  - 0 (Chemotactic Factors)
RN  - 11062-77-4 (Superoxides)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (rac GTP-Binding Proteins)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Aspergillus/immunology
MH  - Calcium-Calmodulin-Dependent Protein Kinases/metabolism
MH  - Chemotactic Factors/pharmacology
MH  - Cytoskeleton/physiology
MH  - Enzyme Activation
MH  - GTP Phosphohydrolases/deficiency/*physiology
MH  - GTP-Binding Proteins/deficiency/*physiology
MH  - Hematopoiesis/physiology
MH  - Hematopoietic Stem Cells/*physiology
MH  - Humans
MH  - Immunity/*physiology
MH  - Mice
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - *Mitogen-Activated Protein Kinases
MH  - Neutrophils/*physiology
MH  - Phosphorylation
MH  - Superoxides/metabolism
MH  - p38 Mitogen-Activated Protein Kinases
MH  - rac GTP-Binding Proteins
EDAT- 1999/03/11 00:00
MHDA- 1999/03/11 00:01
CRDT- 1999/03/11 00:00
PHST- 1999/03/11 00:00 [pubmed]
PHST- 1999/03/11 00:01 [medline]
PHST- 1999/03/11 00:00 [entrez]
AID - S1074-7613(00)80019-9 [pii]
AID - 10.1016/s1074-7613(00)80019-9 [doi]
PST - ppublish
SO  - Immunity. 1999 Feb;10(2):183-96. doi: 10.1016/s1074-7613(00)80019-9.