PMID- 10070062
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190205
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 276
IP  - 3
DP  - 1999 Mar
TI  - TAFII250, Egr-1, and D-type cyclin expression in mice and neonatal rat
      cardiomyocytes treated with doxorubicin.
PG  - H803-14
LID - 10.1152/ajpheart.1999.276.3.H803 [doi]
AB  - Differential display identified that gene fragment HA220 homologous to the
      transcriptional activator factor II 250 (TAFII250) gene, or CCG1, was increased
      in hypertrophied rodent heart. To determine whether TAFII250 gene expression is
      modified after cardiac damage, we measured TAFII250 expression in vivo in mouse
      hearts after injection of the cardiotoxic agent doxorubicin (DXR) and in vitro in
      DXR-treated isolated rat neonatal cardiomyocytes. In vivo atrial natriuretic
      factor (ANF), beta-myosin heavy chain (beta-MHC), Egr-1, and TAFII250 expression 
      increased with dose and time after a single DXR injection, but only ANF and
      beta-MHC expression were increased after multiple injections. After DXR treatment
      of neonatal cardiomyocytes we found decreased ANF, alpha-MHC, Egr-1, and TAFII250
      expression. Expression of the TAFII250-regulated genes, the D-type cyclins, was
      increased after a single injection in adult mice and was decreased in DXR-treated
      cardiomyocytes. Thus expression of Erg-1, TAFII250, and the D-type cyclins is
      modulated after cardiotoxic damage in adult and neonatal heart.
FAU - Saadane, N
AU  - Saadane N
AD  - Lady Davis Institute for Medical Research, Montreal H3T 1E2, Montreal, Quebec,
      Canada H3A 1A3.
FAU - Alpert, L
AU  - Alpert L
FAU - Chalifour, L E
AU  - Chalifour LE
LA  - eng
SI  - GENBANK/AF081115
SI  - GENBANK/AF081116
SI  - GENBANK/AF081117
SI  - GENBANK/AH007553
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Cyclin D)
RN  - 0 (Cyclins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Early Growth Response Protein 1)
RN  - 0 (Egr1 protein, mouse)
RN  - 0 (Egr1 protein, rat)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (TATA-Binding Protein Associated Factors)
RN  - 0 (Transcription Factor TFIID)
RN  - 0 (Transcription Factors)
RN  - 63231-63-0 (RNA)
RN  - 80168379AG (Doxorubicin)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.7.11.1 (TATA-binding protein associated factor 250 kDa)
SB  - IM
MH  - Amino Acid Sequence/genetics
MH  - Animals
MH  - Animals, Newborn/genetics
MH  - Cardiomegaly/physiopathology
MH  - Cyclin D
MH  - Cyclins/*genetics
MH  - DNA-Binding Proteins/*genetics/physiology
MH  - Doxorubicin/*pharmacology
MH  - Early Growth Response Protein 1
MH  - Gene Expression/*drug effects/physiology
MH  - Gene Expression Regulation/physiology
MH  - Heart/drug effects/*physiopathology
MH  - Histone Acetyltransferases
MH  - *Immediate-Early Proteins
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Molecular Sequence Data
MH  - Myocardium/metabolism/pathology
MH  - Nuclear Proteins/*genetics/physiology
MH  - RNA/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *TATA-Binding Protein Associated Factors
MH  - Time Factors
MH  - *Transcription Factor TFIID
MH  - Transcription Factors/*genetics
EDAT- 1999/03/10 00:00
MHDA- 1999/03/10 00:01
CRDT- 1999/03/10 00:00
PHST- 1999/03/10 00:00 [pubmed]
PHST- 1999/03/10 00:01 [medline]
PHST- 1999/03/10 00:00 [entrez]
AID - 10.1152/ajpheart.1999.276.3.H803 [doi]
PST - ppublish
SO  - Am J Physiol. 1999 Mar;276(3):H803-14. doi: 10.1152/ajpheart.1999.276.3.H803.