PMID- 10069812
OWN - NLM
STAT- MEDLINE
DCOM- 19990401
LR  - 20181113
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 10
IP  - 3
DP  - 1999 Mar
TI  - Cytoplasmic dynein heavy chain 1b is required for flagellar assembly in
      Chlamydomonas.
PG  - 693-712
AB  - A second cytoplasmic dynein heavy chain (cDhc) has recently been identified in
      several organisms, and its expression pattern is consistent with a possible role 
      in axoneme assembly. We have used a genetic approach to ask whether cDhc1b is
      involved in flagellar assembly in Chlamydomonas. Using a modified PCR protocol,
      we recovered two cDhc sequences distinct from the axonemal Dhc sequences
      identified previously. cDhc1a is closely related to the major cytoplasmic Dhc,
      whereas cDhc1b is closely related to the minor cDhc isoform identified in sea
      urchins, Caenorhabditis elegans, and Tetrahymena. The Chlamydomonas cDhc1b
      transcript is a low-abundance mRNA whose expression is enhanced by
      deflagellation. To determine its role in flagellar assembly, we screened a
      collection of stumpy flagellar (stf) mutants generated by insertional mutagenesis
      and identified two strains in which portions of the cDhc1b gene have been
      deleted. The two mutants assemble short flagellar stumps (<1-2 micrometer) filled
      with aberrant microtubules, raft-like particles, and other amorphous material.
      The results indicate that cDhc1b is involved in the transport of components
      required for flagellar assembly in Chlamydomonas.
FAU - Porter, M E
AU  - Porter ME
AD  - Department of Cell Biology and Neuroanatomy, University of Minnesota Medical
      School, Minneapolis, Minnesota 55455, USA. mary-p@biosci.cbws.umn.edu
FAU - Bower, R
AU  - Bower R
FAU - Knott, J A
AU  - Knott JA
FAU - Byrd, P
AU  - Byrd P
FAU - Dentler, W
AU  - Dentler W
LA  - eng
SI  - GENBANK/AJ132478
SI  - GENBANK/AJ132799
SI  - GENBANK/AJ132800
SI  - GENBANK/AJ132801
GR  - R01 GM055667/GM/NIGMS NIH HHS/United States
GR  - R37 GM055667/GM/NIGMS NIH HHS/United States
GR  - GM-32556/GM/NIGMS NIH HHS/United States
GR  - GM-55667/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - EC 3.6.4.2 (Dyneins)
SB  - IM
MH  - Alleles
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chlamydomonas/*physiology
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - Cytoplasm/*metabolism
MH  - Dyneins/*genetics/*metabolism
MH  - Flagella/*metabolism/pathology
MH  - Gene Expression Regulation
MH  - Molecular Sequence Data
MH  - Mutagenesis
MH  - Mutation
MH  - Polymerase Chain Reaction/methods
MH  - Polymorphism, Restriction Fragment Length
MH  - Sequence Analysis
MH  - Sequence Homology, Amino Acid
MH  - Transcription, Genetic
PMC - PMC25196
EDAT- 1999/03/09 00:00
MHDA- 1999/03/09 00:01
CRDT- 1999/03/09 00:00
PHST- 1999/03/09 00:00 [pubmed]
PHST- 1999/03/09 00:01 [medline]
PHST- 1999/03/09 00:00 [entrez]
AID - 10.1091/mbc.10.3.693 [doi]
PST - ppublish
SO  - Mol Biol Cell. 1999 Mar;10(3):693-712. doi: 10.1091/mbc.10.3.693.